Plasmid-mediated carbapenem-hydrolyzing enzyme KPC-2 and ArmA 16S rRNA methylase conferring high-level aminoglycoside resistance in carbapenem-resistant Enterobacter cloacae in China

被引：56

作者：

Wu, Qiong ^{[1
]}

Liu, Qingzhong ^{[1
]}

Han, Lizhong ^{[1
]}

Sun, Jingyong ^{[1
]}

Ni, Yuxing ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Clin Microbiol, Shanghai 200025, Peoples R China

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2010年 / 66卷 / 03期

关键词：

Carbapenem; Aminoglycoside; Resistance; 16S rRNA methylase; Enterobacter cloacae; BETA-LACTAMASE; KLEBSIELLA-PNEUMONIAE; TIGECYCLINE; EMERGENCE; IMIPENEM;

D O I：

10.1016/j.diagmicrobio.2009.10.003

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The emergence and spread of carbapenem-resistant Enterobacteriaceae in the world are a major concern. We investigated 5 isolates of Enterobacter cloacae that were resistant to all clinically available antimicrobial agents, except polymyxin B. The MICs of imipenem and aminoglycosides were >32 and >256 mg/L. respectively. All of the isolates produced 5 extended-spectrum beta-lactamase (ESBLs) with pIs of 5.4 (TEM-1), 6.7 (KPC-2), 8.2 (SHV-12), 8.4 (CTX-M-14), and ArmA 16S rRNA methylase. bla(KPC-2) was located on a large nonconjugative plasmid, whereas armA was located on another conjugative plasmid. Although carbapenem-resistant Enterobacteriaceae remains rare, the emergence of this group of organism merits monitoring. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：326 / 328

页数：3

共 20 条

[1]

[Anonymous], 2012, Molecular Cloning: A Laboratory Manual

[2] Linkage of acquired quinolone resistance (qnrS1) and metallo-β-lactamase (blaVIM-1) genes in multiple species of Enterobacteriaceae from Bolzano, Italy [J].

Aschbacher, Richard ;

Doumith, Michel ;

Livermore, David M. ;

Larcher, Clara ;

Woodford, Neil .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2008, 61 (03) :515-523

[3] Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC beta-lactamase, and the loss of an outer membrane protein [J].

Bradford, PA ;

Urban, C ;

Mariano, N ;

Projan, SJ ;

Rahal, JJ ;

Bush, K .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (03) :563-569

[4] Antimicrobial activities of tigecycline and other broad-spectrum antimicrobials tested against serine carbapenemase- and metallo-β-lactamase-producing enterobactefiaceae:: Report from the SENTRY antimicrobial surveillance program [J].

Castanheira, Mariana ;

Sader, Helio S. ;

Deshpande, Lalitagauri M. ;

Fritsche, Thomas R. ;

Jones, Ronald N. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (02) :570-573

[5] High prevalence of the plasmid-mediated quinolone resistance determinant qnrA in multidrug-resistant Enterobacteriaceae from blood cultures in Liverpool, UK [J].

Corkill, JE ;

Anson, JJ ;

Hart, CA .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2005, 56 (06) :1115-1117

[6] Emergence of serine carbapenemases (KPC and SME) among clinical strains of Enterobacteriaceae isolated in the United States Medical Centers: Report from the MYSTIC Program (1999-2005) [J].

Deshpande, Lalitagauri M. ;

Rhomberg, Paul R. ;

Sader, Helio S. ;

Jones, Ronald N. .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2006, 56 (04) :367-372

[7] Plasmid-mediated high-level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation [J].

Galimand, M ;

Courvalin, P ;

Lambert, T .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (08) :2565-2571

[8] Tigecycline: in-vitro performance as a predictor of clinical efficacy [J].

Hawkey, P. ;

Finch, R. .

CLINICAL MICROBIOLOGY AND INFECTION, 2007, 13 (04) :354-362

[9] Occurrence of a new metallo-β-lactamase IMP-4 carried on a conjugative plasmid in Citrobacter youngae from the People's Republic of China [J].

Hawkey, PM ;

Xiong, JH ;

Ye, HF ;

Li, HY ;

M'Zali, FH .

FEMS MICROBIOLOGY LETTERS, 2001, 194 (01) :53-57

[10] Plasmid-mediated carbapenem-hydrolyzing enzyme KPC-2 in an Enterobacter sp. [J].

Hossain, A ;

Ferraro, MJ ;

Pino, RM ;

Dew, RB ;

Moland, ES ;

Lockhart, TJ ;

Thomson, KS ;

Goering, RV ;

Hanson, ND .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (11) :4438-4440

← 1 2 →