Cleaving DNA with DNA

被引:224
作者
Carmi, N [1 ]
Balkhi, SR [1 ]
Breaker, RR [1 ]
机构
[1] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
关键词
D O I
10.1073/pnas.95.5.2233
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A DNA structure is described that can cleave single-stranded DNA oligonucleotides in the presence of ionic copper. This "deoxyribozyme" can self-cleave or can operate as a bimolecular complex that simultaneously makes use of duplex and tripler interactions to bind and cleave separate DNA substrates. Bimolecular deoxyribozyme-mediated strand scission proceeds with a k(obs) of 0.2 min(-1), whereas the corresponding uncatalyzed reaction could not be detected. The duplex and tripler recognition domains can be altered, making possible the targeted cleavage of single-stranded DNAs with different nucleotide sequences. Several small synthetic DNAs were made to function as simple "restriction enzymes" for the site-specific cleavage of single-stranded DNA.
引用
收藏
页码:2233 / 2237
页数:5
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