Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography

被引：173

作者：

Gimeno, E ^{[1
]}

Castellote, AI ^{[1
]}

Lamuela-Raventós, RM ^{[1
]}

de la Torre, MC ^{[1
]}

López-Sabater, MC ^{[1
]}

机构：

[1] Univ Barcelona, Fac Farm, Ctr Referencia Tecnol Aliments, CeRTA,Dpt Nutr Bromatol, E-08028 Barcelona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 881卷 / 1-2期

关键词：

oils; food analysis; vitamins; tocopherols;

D O I：

10.1016/S0021-9673(00)00219-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (ol-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol-water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%). (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：251 / 254

页数：4

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