Biosynthesis of terpenoids:: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase from tomato

被引:96
作者
Rohdich, F
Wungsintaweekul, J
Lüttgen, H
Fischer, M
Eisenreich, W
Schuhr, CA
Fellermeier, M
Schramek, N
Zenk, MH
Bacher, A
机构
[1] Tech Univ Munich, Lehrstuhl Organ Chem & Biochem, D-85747 Garching, Germany
[2] Univ Halle Wittenberg, Biozentrum Pharm, D-06120 Halle, Germany
关键词
D O I
10.1073/pnas.140209197
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The putative catalytic domain (residues 81-401) of a predicted tomato protein with similarity to 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase of Escherichia coli was expressed in a recombinant E. coli strain. The protein was purified to homogeneity and was shown to catalyze the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2-C-methyl-D-erythritol at a rate of 33 mu mol.mg(-1) min(-1). The structure of the reaction product, 4-diphosphocytidyl-2-C-methyl-D-erythrit 2-phosphate, was established by NMR spectroscopy. Divalent metal ions, preferably Mg2+, are required for activity. Neither the tomato enzyme nor the E. coli ortholog catalyzes the phosphorylation of isopentenyl monophosphate.
引用
收藏
页码:8251 / 8256
页数:6
相关论文
共 30 条
[1]  
[Anonymous], 1999, COMPREHENSIVE NATURA, DOI DOI 10.1016/B978-0-08-091283-7.00043-6
[2]   SOME NEW ASPECTS OF ISOPRENOID BIOSYNTHESIS IN PLANTS - A REVIEW [J].
BACH, TJ .
LIPIDS, 1995, 30 (03) :191-202
[3]   STEROL MOLECULE - STRUCTURE, BIOSYNTHESIS, AND FUNCTION [J].
BLOCH, K .
STEROIDS, 1992, 57 (08) :378-383
[4]  
Bochar D.A., 1999, Comprehensive Natural Products Chemistry, VVolume 2, P15
[5]   Dedicated roles of plastid transketolases during the early onset of isoprenoid biogenesis in pepper fruits [J].
Bouvier, F ;
d'Harlingue, A ;
Suire, C ;
Backhaus, RA ;
Camara, B .
PLANT PHYSIOLOGY, 1998, 117 (04) :1423-1431
[6]  
BROERS STJ, 1994, THESIS EIDGENOSSICHE
[7]  
BULLOCK WO, 1987, BIOTECHNIQUES, V5, P376
[8]   The deoxyxylulose phosphate pathway of terpenoid biosynthesis in plants and microorganisms [J].
Eisenreich, W ;
Schwarz, M ;
Cartayrade, A ;
Arigoni, D ;
Zenk, MH ;
Bacher, A .
CHEMISTRY & BIOLOGY, 1998, 5 (09) :R221-R233
[9]   Biosynthesis of terpenoids:: YgbB protein converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate to 2C-methyl-D-erythritol 2,4-cyclodiphosphate [J].
Herz, S ;
Wungsintaweekul, J ;
Schuhr, CA ;
Hecht, S ;
Lüttgen, H ;
Sagner, S ;
Fellermeier, M ;
Eisenreich, W ;
Zenk, MH ;
Bacher, A ;
Rohdich, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (06) :2486-2490
[10]   Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs [J].
Jomaa, H ;
Wiesner, J ;
Sanderbrand, S ;
Altincicek, B ;
Weidemeyer, C ;
Hintz, M ;
Türbachova, I ;
Eberl, M ;
Zeidler, J ;
Lichtenthaler, HK ;
Soldati, D ;
Beck, E .
SCIENCE, 1999, 285 (5433) :1573-1576