The specific expression of three novel splice variant forms of human metalloprotease-like disintegrin-like cysteine-rich protein 2 gene in brain tissues and gliomas

被引:15
作者
Harada, T
Nishie, A
Torigoe, K
Ikezaki, K
Shono, T
Maehara, Y
Kuwano, M
Wada, M
机构
[1] Kyushu Univ, Dept Biochem & Mol Biol, Higashi Ku, Fukuoka 8128582, Japan
[2] Kyushu Univ, Dept Neurosurg, Higashi Ku, Fukuoka 8128582, Japan
[3] Kyushu Univ, Grad Sch Med Sci, Dept Surg, Higashi Ku, Fukuoka 8128582, Japan
来源
JAPANESE JOURNAL OF CANCER RESEARCH | 2000年 / 91卷 / 10期
关键词
MDC; ADAM; alternative splicing; MDR region;
D O I
10.1111/j.1349-7006.2000.tb00877.x
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We have previously identified 67 exons on a yeast artificial chromosome contig spanning 1.5 Mb around the multidrug resistance 1 gene region of human chromosome 7q21.1, In this study, we identified three novel cytoplasmic variants (MDC2-gamma, MDC2-delta, and MDC2-epsilon) of the human metalloprotease-like disintegrin-like cysteine-rich protein 2 (MDC2) among these exons by screening a human brain cDNA library and also by using a reverse transcription polymerase chain reaction, Genomic sequence analysis strongly supported the idea that the variations in the cytoplasmic domain were generated by alternative splicing. The expression of MDC2 variant forms in human brain tissue and gliomas was examined by reverse transcription polymerase chain reaction and RNase protection assay. MDC2-epsilon was expressed only in the cortical and hippocampal regions in human brain, but not in gliomas, In contrast, MDC2-gamma was a major form expressed in human gliomas, Specific expression of these cytoplasmic variants of MDC2 in human brain and its malignancies is discussed.
引用
收藏
页码:1001 / 1006
页数:6
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