Validation of the postimplantation rat whole-embryo culture test in the international ECVAM validation study on three in vitro embryotoxicity tests

被引：92

作者：

Piersma, AH

Genschow, E

Verhoef, A

Spanjersberg, MQI

Brown, NA

Brady, M

Burns, A

Clemann, N

Seiler, A

Spielmann, H

机构：

[1] BfR, Ctr Documentat & Evaluat Alternat Methods Anim Ex, ZEBET, D-12277 Berlin, Germany

[2] Natl Inst Publ Hlth & Environm, RIVM, NL-3720 BA Bilthoven, Netherlands

[3] Univ London St Georges Hosp, Sch Med, London SW17 0RE, England

[4] Novartis Pharm AG, Basel, Switzerland

[5] BfR, Fed Inst Risk Assessment, Berlin, Germany

[6] Syngenta Cent Toxicol Lab, Macclesfield, Cheshire, England

来源：

ATLA-ALTERNATIVES TO LABORATORY ANIMALS | 2004年 / 32卷 / 03期

关键词：

ECVAM; in vitro embryotoxicity test; in vitro toxicology; prediction model; prevalidation; WEC test; whole-embryo culture test;

D O I：

10.1177/026119290403200307

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A detailed report is presented on the performance of the postimplantation rat whole-embryo culture (WEC) test in a European Centre for the Validation of Alternative Methods (ECVAM)-sponsored formal validation study on three in vitro tests for embryotoxicity. Twenty coded test chemicals, classified as non-embryotoxic, weakly embryotoxic or strongly embryotoxic on the basis of their in vivo effects in animals and/or humans, were tested in four laboratories. The outcome showed that the WEC test can be considered to be a scientifically validated test, which is ready for consideration for use in assessing the embryotoxic potentials of chemicals for regulatory purposes.

引用

页码：275 / 307

页数：33

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