Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides

被引:100
作者
Tullman-Ercek, Danielle
DeLisa, Matthew P.
Kawarasaki, Yasuaki
Iranpour, Pooya
Ribnicky, Brian
Palmer, Tracy
Georgiou, George
机构
[1] Univ Texas, Dept Chem, Austin, TX 78712 USA
[2] Univ Texas, Dept Biomed Engn, Austin, TX 78712 USA
[3] Univ Texas, Inst Cell & Mol Biol, Austin, TX 78712 USA
[4] Cornell Univ, Sch Chem & Biomol Engn, Ithaca, NY 14853 USA
[5] John Innes Ctr Plant Sci Res, Dept Mol Microbiol, Norwich NR4 7UH, Norfolk, England
[6] Univ E Anglia, Sch Biol Sci, Norwich NR4 7TJ, Norfolk, England
基金
英国医学研究理事会;
关键词
D O I
10.1074/jbc.M610507200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Escherichia coli genome encodes at least 29 putative signal peptides containing a twin arginine motif characteristic of proteins exported via the twin arginine translocation (Tat) pathway. Fusions of the putative Tat signal peptides plus six to eight amino acids of the mature proteins to three reporter proteins (short-lived green fluorescent protein, maltose-binding protein (MBP), and alkaline phosphatase) and also data from the cell localization of epitope-tagged full-length proteins were employed to determine the ability of the 29 signal peptides to direct export through the Tat pathway, through the general secretory pathway (Sec), or through both. 27/29 putative signal peptides could export one or more reporter proteins through Tat. Of these, I I signal peptides displayed Tat specificity in that they could not direct the export of Sec-only reporter proteins. The rest (16/27) were promiscuous and were capable of directing export of the appropriate reporter either via Tat (green fluorescent protein, MBP) or via Sec (PhoA, MBP). Mutations that conferred a >=+1 charge to the N terminus of the mature protein abolished or drastically reduced routing through the Sec pathway without affecting the ability to export via the Tat pathway. These experiments demonstrate that the charge of the mature protein N terminus affects export promiscuity, independent of the effect of the folding state of the mature protein.
引用
收藏
页码:8309 / 8316
页数:8
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