Development of 16S rRNA-based probes for the Coriobacterium group and the Atopobium cluster and their application for enumeration of Coriobacteriaceae in human feces from volunteers of different age groups

被引：247

作者：

Harmsen, HJM

Wildeboer-Veloo, ACM

Grijpstra, J

Knol, J

Degener, JE

Welling, GW

机构：

[1] Univ Groningen, Dept Med Microbiol, NL-9700 RB Groningen, Netherlands

[2] Num Res BV, NL-6700 CA Wageningen, Netherlands

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 10期

关键词：

D O I：

10.1128/AEM.66.10.4523-4527.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two 16S rRNA-targeted probes were developed: one for the Coriobacterium group and the other for the Atopobium cluster (which comprises most of the Coriobacteriaceae species, including the Coriobacterium group). The new probes were based on sequences of three new Coriobacteriaceae strains isolated from human feces and clinical material and sequences from databases. Application of the probes to fecal samples showed that formula-fed infants had higher numbers of Coriobacterium group cells in their feces than breast-fed infants. In addition, based on the presented results, it is hypothesized that with the increasing age of a person, the diversity of Atopobium cluster species present in the feces increases.

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收藏

页码：4523 / 4527

页数：5

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