Transgene and transposon silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA helicase

被引：226

作者：

Wu-Scharf, D ^{[1
]}

Jeong, BR ^{[1
]}

Zhang, CM ^{[1
]}

Cerutti, H ^{[1
]}

机构：

[1] Univ Nebraska, Sch Biol Sci & Plant Sci Initiat, Beadle Ctr E211, Lincoln, NE 68588 USA

来源：

SCIENCE | 2000年 / 290卷 / 5494期

关键词：

D O I：

10.1126/science.290.5494.1159

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families, Mut6 encodes a protein that is highly homologous to RNA helicases of the DEAH-box family, This protein is necessary for the degradation of certain aberrant RNAs, such as improperly processed transcripts, which are often produced by transposons and some transgenes.

引用

页码：1159 / 1162

页数：4

共 29 条

[1] The yeast exosome and human PM-Scl are related complexes of 3′→5′ exonucleases
Allmang, C
Petfalski, E
Podtelejnikov, A
Mann, M
Tollervey, D
Mitchell, P
[J]. GENES & DEVELOPMENT, 1999, 13 (16) : 2148 - 2158
[2] RNA interference: genetic wand and genetic watchdog
Bosher, JM
Labouesse, M
[J]. NATURE CELL BIOLOGY, 2000, 2 (02) : E31 - E36
[3] Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas
Cerutti, H
Johnson, AM
Gillham, NW
Boynton, JE
[J]. PLANT CELL, 1997, 9 (06) : 925 - 945
[4] Cerutti H, 1997, GENETICS, V145, P97
[5] Posttranscriptional gene silencing in Neurospora by a RecQ DNA helicase
Cogoni, C
Macino, G
[J]. SCIENCE, 1999, 286 (5448) : 2342 - 2344
[6] An RNA-Dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus
Dalmay, T
Hamilton, A
Rudd, S
Angell, S
Baulcombe, DC
[J]. CELL, 2000, 101 (05) : 543 - 553
[7] STRUCTURE AND INHERITANCE OF SENSE AND ANTISENSE TRANSCRIPTS FROM A TRANSPOSON IN THE GREEN-ALGA CHLAMYDOMONAS-REINHARDTII
DAY, A
ROCHAIX, JD
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1991, 218 (02) : 273 - 291
[8] FERRIS PJ, 1989, GENETICS, V122, P363
[9] RNA-triggered gene silencing
Fire, A
[J]. TRENDS IN GENETICS, 1999, 15 (09) : 358 - 363
[10] Genetic requirements for inheritance of RNAi in C-elegans
Grishok, A
Tabara, H
Mello, CC
[J]. SCIENCE, 2000, 287 (5462) : 2494 - 2497

← 1 2 3 →