Multiplex PCR assay for identification of human diarrheagenic Escherichia coli

被引：161

作者：

Toma, C ^{[1
]}

Lu, Y

Higa, N

Nakasone, N

Chinen, I

Baschkier, A

Rivas, M

Iwanaga, M

机构：

[1] Univ Ryukyus, Fac Med, Dept Bacteriol, Nishihara, Okinawa 9030215, Japan

[2] Inst Nacl Enfermedades Infecciosas, Serv Fisopatogenia, ANLIS Dr Carlos G Malbran, RA-1281 Buenos Aires, DF, Argentina

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 06期

关键词：

D O I：

10.1128/JCM.41.6.2669-2671.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube.

引用

页码：2669 / 2671

页数：3

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