RecA protein-mediated irreversible fixation of an oligodeoxyribonucleotide to specific site in DNA

被引：3

作者：

Golub, EI

Glazer, PM

Ward, DC

Radding, CM

机构：

[1] Yale University, School of Medicine, Department of Genetics, New Haven, CT 06510

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1996年 / 351卷 / 02期

关键词：

D O I：

10.1016/0027-5107(95)00212-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

RecA protein can polymerize on an oligodeoxyribonucleotide to form a filament that finds its homologous sequence in double-stranded DNA. When such an oligonucleotide is linked to psoralen, a photoactivatable DNA intercalator, it irreversibly binds to the homologous site in double-stranded DNA as a result of psoralen photoadduct formation at thymidines. The relative efficiency of specific vs. non-specific binding of an oligonucleotide depended upon the ratio of psoralenated oligonucleotide to total DNA. Na+ ions at concentrations greater than 50 mM eliminated specific binding. Under optimal conditions, the probability of binding of an 80-mer oligonucleotide to a specific site was > 10(5) times greater than that of binding to any single non-specific site. Under the conditions described, RecA-mediated photoadduction was equally efficient with superhelical and linear double-stranded DNA.

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页码：117 / 124

页数：8

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