Investigations on the behaviour of acidic, basic and neutral compounds in capillary electrochromatography on a mixed-mode stationary phase

被引：25

作者：

Klampfl, CW ^{[1
]}

Hilder, EF ^{[1
]}

Haddad, PR ^{[1
]}

机构：

[1] Univ Tasmania, Sch Chem, Separat Sci Grp, Hobart, Tas 7001, Australia

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 888卷 / 1-2期

基金：

奥地利科学基金会;

关键词：

electrochromatography; mixed-mode retention; anion exchange; stationary phases; CEC;

D O I：

10.1016/S0021-9673(00)00613-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This work describes the separation of acidic, basic and neutral organic compounds as well as inorganic anions in a single run by capillary electrochromatography employing a stationary phase which exhibits both strong anion-exchange and reversed-phase chromatographic characteristics. The positive surface charge of this stationary phase provided a substantial anodic electroosmotic flaw. The analytes were separated by a mixed-mode mechanism which comprised chromatographic interactions (hydrophobic interactions, ion-exchange) as well as electrophoretic migration. The influence of ion-exchange and hydrophobic interactions on the retention/migration of the analytes could be manipulated by varying the concentration of a competing ion and/or the amount of organic modifier present in the background electrolyte. Additionally the effects of pH changes on both the chromatographic interactions as well as the electrophoretic migration of the analyles were investigated. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：267 / 274

页数：8

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