学术探索
学术期刊
新闻热点
数据分析
智能评审

Development of a method for determination of the malondialdehyde-deoxyguanosine adduct in urine using liquid chromatography-tandem mass spectrometry

被引：16
作者：
Otteneder, M
Daniels, JS
Voehler, M
Marnett, LJ
机构：
[1] Vanderbilt Univ, Dept Biochem, Nashville, TN 37232 USA
[2] Vanderbilt Univ, Dept Chem, Ctr Mol Toxicol, Nashville, TN 37232 USA
[3] Vanderbilt Univ, Vanderbilt Ingram Comprehens Canc Ctr, Nashville, TN 37232 USA
来源：
ANALYTICAL BIOCHEMISTRY | 2003年 / 315卷 / 02期
关键词：
malondialdehyde; DNA adduct; pyrimidopurinone; liquid chromatography-mass spectrometry;
D O I：
10.1016/S0003-2697(02)00697-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A procedure is described for the quantification of the major malondialdehyde deoxyguanosine adduct, pyrimido[1,2-alpha]purin-10(3H)-one-deoxyribose (M1GdR) in urine. M1GdR is isolated from urine by a combination Of C-18 solid-phase extraction and immunoaffinity chromatography. Sodium borohydride treatment reduces M1GdR to the 5,6-dihydro derivative, which is quantified by liquid chromatography-mass spectrometry. Authentic [7,9-N-15,8-C-13] M1GdR is added to urine as an internal standard. A detection limit of 50 fmol M1GdR/ml urine is achieved starting with 5 ml of urine. Analysis of urine samples from control rats or rats treated with CCl4 indicates that the levels of M1GdR are below the detection limit of the assay. This method is easily adaptable to the analysis of M1GdR in DNA samples or biological fluids. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：147 / 151
页数：5
相关论文
共 20 条
[1]   UNEQUIVOCAL DEMONSTRATION THAT MALONDIALDEHYDE IS A MUTAGEN [J].
BASU, AK ;
MARNETT, LJ .
CARCINOGENESIS, 1983, 4 (03) :331-333
[2]  
BERNHEIM F, 1948, J BIOL CHEM, V174, P257
[3]   DETECTION OF ENDOGENOUS MALONDIALDEHYDE-DEOXYGUANOSINE ADDUCTS IN HUMAN LIVER [J].
CHAUDHARY, AK ;
NOKUBO, M ;
REDDY, GR ;
YEOLA, SN ;
MORROW, JD ;
BLAIR, IA ;
MARNETT, LJ .
SCIENCE, 1994, 265 (5178) :1580-1582
[4]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION FOR DETERMINATION OF THE MAJOR MALONDIALDEHYDE GUANINE ADDUCT [J].
GODA, Y ;
MARNETT, LJ .
CHEMICAL RESEARCH IN TOXICOLOGY, 1991, 4 (05) :520-524
[5]  
HAMBERG M, 1967, J BIOL CHEM, V242, P5336
[6]  
JAJOO HK, 1992, CHEM RES TOXICOL, V6, P870
[7]   Comparison of DNA adduct levels associated with oxidative stress in human pancreas [J].
Kadlubar, FF ;
Anderson, KE ;
Häussermann, S ;
Lang, NP ;
Barone, GW ;
Thompson, PA ;
MacLeod, SL ;
Chou, MW ;
Mikhailova, M ;
Plastaras, J ;
Marnett, LJ ;
Nair, J ;
Velic, I ;
Bartsch, H .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1998, 405 (02) :125-133
[8]   Determination of malondialdehyde-induced DNA damage in human tissues using an immunoslot blot assay [J].
Leuratti, C ;
Singh, R ;
Lagneau, C ;
Farmer, PB ;
Plastaras, JP ;
Marnett, LJ ;
Shuker, DEG .
CARCINOGENESIS, 1998, 19 (11) :1919-1924
[9]   Analysis of the malondialdehyde-2'-deoxyguanosine adduct pyrimidopurinone in human leukocyte DNA by gas chromatography electron capture negative chemical ionization mass spectrometry [J].
Rouzer, CA ;
Chaudhary, AK ;
Nokubo, M ;
Ferguson, DM ;
Reddy, GR ;
Blair, IA ;
Marnett, LJ .
CHEMICAL RESEARCH IN TOXICOLOGY, 1997, 10 (02) :181-188
[10]   Synthesis of oligonucleotides containing the alkali-labile pyrimidopurinone adduct, M1G [J].
Schnetz-Boutaud, NC ;
Mao, H ;
Stone, MP ;
Marnett, LJ .
CHEMICAL RESEARCH IN TOXICOLOGY, 2000, 13 (02) :90-95
12