A convenient microwave-enhanced solid-phase synthesis of difficult peptide sequences: Case study of Gramicidin A and CSF114(Glc)

被引：54

作者：

Rizzolo, Fabio

Sabatino, Giuseppina

Chelli, Mario

Rovero, Paolo

Papini, Anna Maria

机构：

[1] Univ Florence, Dipartimento Chim Organ Ugo Schiff, I-50019 Sesto Fiorentino, FI, Italy

[2] Univ Florence, CNR IC COM, Polo Sci, I-50019 Sesto Fiorentino, FI, Italy

[3] Univ Florence, Lab Peptide Prot Chem Biol, Polo Sci & Tecnol, I-50019 Sesto Fiorentino, FI, Italy

[4] Univ Florence, Dipartimento Sci Farmaceut, I-50019 Sesto Fiorentino, FI, Italy

来源：

INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS | 2007年 / 13卷 / 1-2期

关键词：

SPPS; SPGS; automatic MW-SPPS; Gramicidin A; CSF114(Glc);

D O I：

10.1007/s10989-006-9066-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Taking into account that microwave technology applied to SPPS is proposed as a valid support to the enhancement of efficiency of coupling reactions in short times, we compared the efficacy of a new automatic monomode microwave instrument versus a traditional automatic peptide synthesizer in the synthesis of different difficult peptide sequences. We verified that microwave-assisted solid phase synthesis is convenient, in terms of yield, purity, and time consumed to prepare two peptides which were previously shown to be difficult to obtain by conventional strategy, i.e., the antibiotic Gramicidin A and a glycopeptide.

引用

页码：203 / 208

页数：6

共 15 条

[1] Developments in peptide and amide synthesis [J].