Human immunodeficiency virus type 1 Vif protein is an integral component of an mRNP complex of viral RNA and could be involved in the viral RNA folding and packaging process
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Zhang, H
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Thomas Jefferson Univ, Jefferson Med Coll, Dorrance H Hamilton Labs, Ctr Human Virol,Div Infect Dis,Dept Med, Philadelphia, PA 19107 USAThomas Jefferson Univ, Jefferson Med Coll, Dorrance H Hamilton Labs, Ctr Human Virol,Div Infect Dis,Dept Med, Philadelphia, PA 19107 USA
Zhang, H
[1
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Pomerantz, RJ
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Thomas Jefferson Univ, Jefferson Med Coll, Dorrance H Hamilton Labs, Ctr Human Virol,Div Infect Dis,Dept Med, Philadelphia, PA 19107 USAThomas Jefferson Univ, Jefferson Med Coll, Dorrance H Hamilton Labs, Ctr Human Virol,Div Infect Dis,Dept Med, Philadelphia, PA 19107 USA
Pomerantz, RJ
[1
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Dornadula, G
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Thomas Jefferson Univ, Jefferson Med Coll, Dorrance H Hamilton Labs, Ctr Human Virol,Div Infect Dis,Dept Med, Philadelphia, PA 19107 USAThomas Jefferson Univ, Jefferson Med Coll, Dorrance H Hamilton Labs, Ctr Human Virol,Div Infect Dis,Dept Med, Philadelphia, PA 19107 USA
Dornadula, G
[1
]
Sun, Y
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Thomas Jefferson Univ, Jefferson Med Coll, Dorrance H Hamilton Labs, Ctr Human Virol,Div Infect Dis,Dept Med, Philadelphia, PA 19107 USAThomas Jefferson Univ, Jefferson Med Coll, Dorrance H Hamilton Labs, Ctr Human Virol,Div Infect Dis,Dept Med, Philadelphia, PA 19107 USA
Sun, Y
[1
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[1] Thomas Jefferson Univ, Jefferson Med Coll, Dorrance H Hamilton Labs, Ctr Human Virol,Div Infect Dis,Dept Med, Philadelphia, PA 19107 USA
Virion infectivity factor (Vif) is a protein encoded by human immunodeficiency virus types 1 and 2 (HIv-1 and -2) and simian immunodeficiency virus, plus other lentiviruses and is essential for viral replication either in vivo or in culture for nonpermissive cells such as peripheral blood lymphoid cells, macrophages, and H9 T cells. Defects in the vif gene affect virion morphology and reverse transcription but not the expression of viral components. It has been shown that Vif colocalizes with Gag in cells and Vif binds to the NCp7 domain of Gag in vitro. However, it seems that Vif is not specifically packaged into virions. The molecular mechanism(s) for Vif remains unknown. In this report, we demonstrate that HIV-1 Vif is an RNA-binding protein and specifically binds to HIV-1 genomic RNA in vitro. Further, Vif binds to HIV-1 RNA in the cytoplasm of virus-producing cells to form a 40S mRNP complex. Coimmunoprecipitation and in vivo UV cross-linking assays indicated that Vif directly interact with HIV-1 RNA in the virus-producing cells. Vif-RNA binding could be displaced by Gag-RNA binding, suggesting that Vif protein in the mRNP complex may mediate viral RNA interaction with HIV-1 Gag precursors. Furthermore, we have demonstrated that these Vif mutants that lose the RNA binding activity in vitro do not support vif-deficient HIV-1 replication in H9 T cells, suggesting that the RNA binding capacity of Vif is important for its function. Further studies regarding Vif-RNA interaction in virus-producing cells will be important for studying the function of Vif in the HIV-1 life cycle.
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HARVARD UNIV,NEW ENGLAND REG PRIMATE RES CTR,SCH MED,SOUTHBOROUGH,MA 01772HARVARD UNIV,NEW ENGLAND REG PRIMATE RES CTR,SCH MED,SOUTHBOROUGH,MA 01772
GIBBS, JS
REGIER, DA
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HARVARD UNIV,NEW ENGLAND REG PRIMATE RES CTR,SCH MED,SOUTHBOROUGH,MA 01772HARVARD UNIV,NEW ENGLAND REG PRIMATE RES CTR,SCH MED,SOUTHBOROUGH,MA 01772
REGIER, DA
DESROSIERS, RC
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HARVARD UNIV,NEW ENGLAND REG PRIMATE RES CTR,SCH MED,SOUTHBOROUGH,MA 01772HARVARD UNIV,NEW ENGLAND REG PRIMATE RES CTR,SCH MED,SOUTHBOROUGH,MA 01772
机构:
RUTGERS STATE UNIV, UNIV MED & DENT NEW JERSEY, ROBERT WOOD JOHNSON MED SCH, PISCATAWAY, NJ 08854 USARUTGERS STATE UNIV, UNIV MED & DENT NEW JERSEY, ROBERT WOOD JOHNSON MED SCH, PISCATAWAY, NJ 08854 USA
Jacobson, A
Peltz, SW
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RUTGERS STATE UNIV, UNIV MED & DENT NEW JERSEY, ROBERT WOOD JOHNSON MED SCH, PISCATAWAY, NJ 08854 USARUTGERS STATE UNIV, UNIV MED & DENT NEW JERSEY, ROBERT WOOD JOHNSON MED SCH, PISCATAWAY, NJ 08854 USA
机构:
HARVARD UNIV,NEW ENGLAND REG PRIMATE RES CTR,SCH MED,SOUTHBOROUGH,MA 01772HARVARD UNIV,NEW ENGLAND REG PRIMATE RES CTR,SCH MED,SOUTHBOROUGH,MA 01772
GIBBS, JS
REGIER, DA
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HARVARD UNIV,NEW ENGLAND REG PRIMATE RES CTR,SCH MED,SOUTHBOROUGH,MA 01772HARVARD UNIV,NEW ENGLAND REG PRIMATE RES CTR,SCH MED,SOUTHBOROUGH,MA 01772
REGIER, DA
DESROSIERS, RC
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机构:
HARVARD UNIV,NEW ENGLAND REG PRIMATE RES CTR,SCH MED,SOUTHBOROUGH,MA 01772HARVARD UNIV,NEW ENGLAND REG PRIMATE RES CTR,SCH MED,SOUTHBOROUGH,MA 01772
机构:
RUTGERS STATE UNIV, UNIV MED & DENT NEW JERSEY, ROBERT WOOD JOHNSON MED SCH, PISCATAWAY, NJ 08854 USARUTGERS STATE UNIV, UNIV MED & DENT NEW JERSEY, ROBERT WOOD JOHNSON MED SCH, PISCATAWAY, NJ 08854 USA
Jacobson, A
Peltz, SW
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机构:
RUTGERS STATE UNIV, UNIV MED & DENT NEW JERSEY, ROBERT WOOD JOHNSON MED SCH, PISCATAWAY, NJ 08854 USARUTGERS STATE UNIV, UNIV MED & DENT NEW JERSEY, ROBERT WOOD JOHNSON MED SCH, PISCATAWAY, NJ 08854 USA