The interface behavior of hemoglobin at carbon nanotube and the detection for H2O2

被引：172

作者：

Zhao, YD ^{[1
]}

Bi, YH

Zhang, WD

Luo, QM

机构：

[1] Huazhong Univ Sci & Technol, Minist Educ, Key Lab Biomed Photon, Wuhan 430074, Peoples R China

[2] Inst Mat Res & Engn, Singapore 117602, Singapore

来源：

TALANTA | 2005年 / 65卷 / 02期

基金：

中国国家自然科学基金;

关键词：

hemoglobin; carbon nanotube; electrochemistry; nanobiotechnique;

D O I：

10.1016/j.talanta.2004.06.028

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Direct electrochemistry of he moglobin (Hb) is observed at carbon nanotube (CNT) interface. The adsorbing Hb can transfer electron directly at CNT interface compared with common carbon material. The heterogeneous electron transfer rate constant k of Hb can be calculated as 0.062 s(-1). the transfer coefficient alpha is 0.21 and the average surface coverage of Hb on CNT surface is 3.58 x 10(-9) +/- 12.7 x 10(-10) in mol/cm(2). It is found that the adsorbing Hb still keeps its catalytic activity to H2O2. This sensor was used to detect H2O2. The apparent Michaelis-Menten constant is calculated as 6.75 x 10(-4) mol L-1. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：489 / 494

页数：6

共 31 条

[1] Preparation and characterization of rare earth orthovanadates for propane oxidative dehydrogenation [J].

Au, CT ;

Zhang, WD ;

Wan, HL .

CATALYSIS LETTERS, 1996, 37 (3-4) :241-246

[2] High specific surface area carbon nanotubes from catalytic chemical vapor deposition process [J].

Bacsa, RR ;

Laurent, C ;

Peigney, A ;

Bacsa, WS ;

Vaugien, T ;

Rousset, A .

CHEMICAL PHYSICS LETTERS, 2000, 323 (5-6) :566-571

[3] Carbon nanotube electrode for oxidation of dopamine [J].

Britto, PJ ;

Santhanam, KSV ;

Ajayan, PM .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1996, 41 (01) :121-125

[4] Direct electron transfer and bioelectrocatalysis of hemoglobin at a carbon nanotube electrode [J].

Cai, CX ;

Chen, J .

ANALYTICAL BIOCHEMISTRY, 2004, 325 (02) :285-292

[5] STUDY OF THE ELECTRODE PROCESS OF HEMOGLOBIN AT A POLYMERIZED AZURE A FILM ELECTRODE [J].

DONG, SJ ;

CHU, QH .

ELECTROANALYSIS, 1993, 5 (02) :135-140

[6] A reagentless nitric oxide biosensor based on hemoglobin-DNA films [J].

Fan, CH ;

Li, GX ;

Zhu, JQ ;

Zhu, DX .

ANALYTICA CHIMICA ACTA, 2000, 423 (01) :95-100

[7] An unmediated hydrogen peroxide sensor based on a hemoglobin-SDS film modified electrode [J].

Fan, CH ;

Suzuki, I ;

Chen, Q ;

Li, GX ;

Anzai, J .

ANALYTICAL LETTERS, 2000, 33 (13) :2631-2644

[8] Effect of dimethyl sulfoxide on the electron transfer reactivity of hemoglobin [J].

Fan, CH ;

Wagner, G ;

Li, GX .

BIOELECTROCHEMISTRY, 2001, 54 (01) :49-51

[9] A SPECTROELECTROCHEMICAL METHOD FOR DIFFERENTIATION OF STERIC AND ELECTRONIC EFFECTS IN HEMOGLOBINS AND MYOGLOBINS [J].

FAULKNER, KM ;

BONAVENTURA, C ;

CRUMBLISS, AL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (23) :13604-13612

[10] Synthesis of single-walled carbon nanotube-Co-MgO composite powders and extraction of the nanotubes [J].

Flahaut, E ;

Peigney, A ;

Laurent, C ;

Rousset, A .

JOURNAL OF MATERIALS CHEMISTRY, 2000, 10 (02) :249-252

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