An efficient fusion expression system for protein and peptide overexpression in Escherichia coli and NMR sample preparation

被引：7

作者：

Cheng, Y ^{[1
]}

Liu, DS ^{[1
]}

Feng, YM ^{[1
]}

Jing, GZ ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

来源：

PROTEIN AND PEPTIDE LETTERS | 2003年 / 10卷 / 02期

关键词：

protein expression; small fusion partner; NMR sample preparation;

D O I：

10.2174/0929866033479112

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An efficient fusion expression system with a small fusion partner, His(6)-tagged N-terminal fragment of staphylococcal nuclease R, has been constructed and tested with two genes. The results show that the system is not only suitable for overexpression of small proteins and peptides but simplifies purification of target proteins and peptides. The study also provides a practical method for preparation of isotope-labeled protein sample for NMR analysis.

引用

页码：175 / 181

页数：7

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