Multispacer typing technique for sequence-based typing of Bartonella quintana

被引:28
作者
Foucault, C
La Scola, B
Lindroos, H
Andersson, SGE
Raoult, D
机构
[1] Univ Mediterranee, Fac Med Marseille, Unite Rickettsies, CNRS UMR 6020,IFR 48, F-13385 Marseille 05, France
[2] Uppsala Univ, Dept Mol Evolut, Evolutionary Biol Ctr, Uppsala, Sweden
关键词
D O I
10.1128/JCM.43.1.41-48.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bartonella quintana is a worldwide fastidious bacterium of the Alphaproteobacteria responsible for bacillary angiomatosis, trench fever, chronic lymphadenopathy, and culture-negative endocarditis. The recent genome sequencing of a B. quintana isolate allowed us to propose a genome-wide sequence-based typing method. To ensure sequence discrimination based on highly polymorphic areas, we amplified and sequenced 34 spacers in a large collection of B. quintana isolates. Six of these exhibited polymorphisms and allowed the characterization of 4 genotypes. However, the strain variants suggested by the noncoding sequences did not correlate with the results of pulsed-field gel electrophoresis (PFGE), which suggested a higher degree of variability. Modification of the PFGE profile of one isolate after nine subcultures confirmed that rearrangement frequencies are high in this species, making PFGE unreliable for epidemiological purposes. The low extent of sequence heterogeneity in the species suggests a recent emergence of this bacterium as a human pathogen. Direct typing of natural samples allowed the identification of a fifth genotype in the DNA extracted from a human body louse collected in Burundi. We have named the typing technique herein described multispacer typing.
引用
收藏
页码:41 / 48
页数:8
相关论文
共 47 条
[41]   Body lice as tools for diagnosis and surveillance of reemerging diseases [J].
Roux, V ;
Raoult, D .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (03) :596-599
[42]   BARTONELLA (ROCHALIMAEA) QUINTANA BACTEREMIA IN INNER-CITY PATIENTS WITH CHRONIC-ALCOHOLISM [J].
SPACH, DH ;
KANTER, AS ;
DOUGHERTY, MJ ;
LARSON, AM ;
COYLE, MB ;
BRENNER, DJ ;
SWAMINATHAN, B ;
MATAR, GM ;
WELCH, DF ;
ROOT, RK ;
STAMM, WE .
NEW ENGLAND JOURNAL OF MEDICINE, 1995, 332 (07) :424-428
[43]   BARTONELLA (ROCHALIMAEA) SPECIES AS A CAUSE OF APPARENT CULTURE-NEGATIVE ENDOCARDITIS [J].
SPACH, DH ;
KANTER, AS ;
DANIELS, NA ;
NOWOWIEJSKI, DJ ;
LARSON, AM ;
SCHMIDT, RA ;
SWAMINATHAN, B ;
BRENNER, DJ .
CLINICAL INFECTIOUS DISEASES, 1995, 20 (04) :1044-1047
[44]   ENDOCARDITIS CAUSED BY ROCHALIMAEA-QUINTANA IN A PATIENT INFECTED WITH HUMAN-IMMUNODEFICIENCY-VIRUS [J].
SPACH, DH ;
CALLIS, KP ;
PAAUW, DS ;
HOUZE, YB ;
SCHOENKNECHT, FD ;
WELCH, DF ;
ROSEN, H ;
BRENNER, DJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (03) :692-694
[45]   RETURN OF TRENCH FEVER [J].
STEIN, A ;
RAOULT, D .
LANCET, 1995, 345 (8947) :450-451
[46]   CLUSTAL-W - IMPROVING THE SENSITIVITY OF PROGRESSIVE MULTIPLE SEQUENCE ALIGNMENT THROUGH SEQUENCE WEIGHTING, POSITION-SPECIFIC GAP PENALTIES AND WEIGHT MATRIX CHOICE [J].
THOMPSON, JD ;
HIGGINS, DG ;
GIBSON, TJ .
NUCLEIC ACIDS RESEARCH, 1994, 22 (22) :4673-4680
[47]   Homologous protection but lack of heterologous-protection by various species and types of Bartonella in specific pathogen-free cats [J].
Yamamoto, K ;
Chomel, BB ;
Kasten, RW ;
Chang, CC ;
Tseggai, T ;
Decker, PR ;
Mackowiak, M ;
Floyd-Hawkins, KA ;
Pedersen, NC .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1998, 65 (2-4) :191-204