Role of E-coli transcription-repair coupling factor mfd in Nun-mediated transcription termination

被引：43

作者：

Washburn, RS

Wang, YS

Gottesman, ME

机构：

[1] Columbia Univ Coll Phys & Surg, Inst Canc Res, New York, NY 10032 USA

[2] Columbia Univ Coll Phys & Surg, Dept Microbiol, New York, NY 10032 USA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2003年 / 329卷 / 04期

关键词：

transcription-repair coupling factor; Mfd; Nun protein; transcription termination;

D O I：

10.1016/S0022-2836(03)00465-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phage HK022 Nun protein excludes phage lambda by binding nascent lambda-nut RNA and inducing termination and transcript release. In contrast, in a purified in vitro system, Nun arrests transcription on lambdaDNA templates without dissociation of the transcription elongation complex (TEC). Our evidence indicates that transcription-repair coupling factor (Mfd) frees Nun-arrested RNA polymerase. The activity of Nun is enhanced in an mfd-null mutant, consistent with prolonged association of Nun with the TEC. Furthermore, expression of lambda nut RNA in the mfd mutant titrates Nun, allowing superinfecting lambda to form plaques. Finally, addition of Mfd releases a Nun-arrested transcription complex in vitro. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：655 / 662

页数：8

共 24 条

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