Sonoreactor-based technology for fast high-throughput proteolytic digestion of proteins

被引：41

作者：

Rial-Otero, R. ^{[1
]}

Carreira, R. J. ^{[1
]}

Cordeiro, F. M. ^{[1
]}

Moro, A. J. ^{[1
]}

Fernandes, L. ^{[1
]}

Moura, I. ^{[1
]}

Capelo, J. L. ^{[1
]}

机构：

[1] Univ Nova Lisboa, REQUIMTE, Dept Quim, Fac Ciencias & Tecnol, P-2829516 Monte De Caparica, Portugal

来源：

JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 02期

关键词：

protein digestion; sonoreactor; MALDI-TOF-MS;

D O I：

10.1021/pr060508m

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fast (120 s) and high-throughput (more than six samples at once) in-gel trypsin digestion of proteins using sonoreactor technology has been achieved. Successful protein identification was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS. Specific identification of the adenylylsulphate reductase alfa subunit from a complex protein mixture from Desulfovibrio desulfuricans ATCC 27774 was done as a proof of the methodology. The new sample treatment is of easy implementation, saves time and money, and can be adapted to online procedures and robotic platforms.

引用

页码：909 / 912

页数：4

共 8 条

[1] Clinical proteomics: From biomarker discovery and cell signaling profiles to individualized personal therapy [J].