Bacterial strain-specific induction of Foxp3+T regulatory cells is protective in murine allergy models

被引：181

作者：

Lyons, A. ^{[2
]}

O'Mahony, D. ^{[2
]}

O'Brien, F. ^{[3
]}

MacSharry, J. ^{[2
]}

Sheil, B. ^{[2
]}

Ceddia, M. ^{[4
]}

Russell, W. M. ^{[4
]}

Forsythe, P. ^{[5
]}

Bienenstock, J. ^{[5
]}

Kiely, B. ^{[2
]}

Shanahan, F. ^{[3
]}

O'Mahony, L. ^{[1
]}

机构：

[1] Swiss Inst Allergy & Asthma Res, SIAF, CH-7270 Davos, Switzerland

[2] Alimentary Hlth Ltd, Cork, Ireland

[3] Natl Univ Ireland Univ Coll Cork, Alimentary Pharmabiot Ctr, Cork, Ireland

[4] Mead Johnson Nutr, Evansville, IN USA

[5] McMaster Univ, Brain Body Inst, Hamilton, ON, Canada

来源：

CLINICAL AND EXPERIMENTAL ALLERGY | 2010年 / 40卷 / 05期

基金：

爱尔兰科学基金会;

关键词：

allergy; commensal microbes; T regulatory cells; PLACEBO-CONTROLLED TRIAL; T-CELLS; ATOPIC DISEASE; ORAL TREATMENT; RETINOIC-ACID; KNOCKOUT MICE; DOUBLE-BLIND; IN-VIVO; KAPPA-B; PROBIOTICS;

D O I：

10.1111/j.1365-2222.2009.03437.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

P>Background The incidence of atopic disease has increased dramatically during recent decades and the potential immunoregulatory influence of the microbiota in these individuals is under investigation. Objective The aim of our study was to identify a bacterial strain that is protective in murine allergy models and to determine if microbial induction of T regulatory cells was associated with protection from allergic inflammation. Methods Three microbes (Bifidobacterium breve AH1205, B. longum AH1206 and Lactobacillus salivarius AH102) of human origin were fed to newborn, adult and germ-free animals. Induction of Foxp3+ T regulatory cells was assessed by flow cytometry. Gene array analysis was performed on Peyer's patches. Strains were also examined for their protective effects in the ovalbumin (OVA) respiratory allergy model and the OVA-cholera toxin dietary allergy model. Results Bifidobacterium longum AH1206 consumption resulted in increased numbers of Foxp3+ T regulatory cells in infant, adult and germ-free animals. B. breve AH1205 induced Foxp3+ T regulatory cell expansion only in infant mice while L. salivarius AH102 did not alter T regulatory cell numbers in any animal model tested. B. longum AH1206 reduced the Peyer's patch gene expression associated with antigen presentation, TLR signalling and cytokine production while increasing the expression of genes associated with retinoic acid metabolism. B. longum AH1206 protected against airway inflammation in OVA-sensitized animals and B. longum AH1206 blocked the induction of IgE to orally administered OVA. Neither B. breve AH1205 nor L. salivarius AH102 had a protective effect in either model. Conclusion Bacterial strain-specific induction of Foxp3+ T regulatory cells in vivo is associated with protection from respiratory and oral allergy. Cite this as: A. Lyons, D. O'Mahony, F. O'Brien, J. MacSharry, B. Sheil, M. Ceddia, W. M.Russell, P. ForsytheJ. Bienenstock, B. Kiely, F. Shanahan and L. O'Mahony, Clinical & Experimental Allergy, 2010 (40) 811-819.

引用

页码：811 / 819

页数：9

共 37 条

[1] Therapeutic manipulation of immune tolerance in allergic disease [J].