Xanthomonas campestris pv. campestris lpsI and lpsJ genes encoding putative proteins with sequence similarity to the α- and β-subunits of 3-oxoacid CoA-transferases are involved in LPS biosynthesis

被引：11

作者：

Steinmann, D ^{[1
]}

Köplin, R ^{[1
]}

Pühler, A ^{[1
]}

Niehaus, K ^{[1
]}

机构：

[1] Univ Bielefeld, Fak Biol, Lehrstuhl Genet, D-33501 Bielefeld, Germany

来源：

ARCHIVES OF MICROBIOLOGY | 1997年 / 168卷 / 06期

关键词：

Xanthomonas campestris pv. campestris; lpsI; lpsJ; 3-oxoacid CoA-transferase; xanthan; lipopolysaccharide;

D O I：

10.1007/s002030050520

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 2.1-kb SmaI-EcoRI DNA fragment upstream of the xanA and xanB genes of Xanthomonas campestris pv. campestris carries two ORFs encoding putative proteins with sequence similarities to the alpha- and beta-subunits of 3-oxoacid-CoA transferases. The two ORFs were termed lpsI and lpsJ because strains carrying appropriate mutations showed an autoagglutination phenotype and because lipopolysaccharides of these mutant strains were altered according to silver-stained polyacrylamide gels. The monosaccharide composition of the exopolysaccharide xanthan produced by lpsI and lpsJ mutants remained unchanged.

引用

页码：441 / 447

页数：7

共 37 条

[11]

HOROWICZ D, 1981, NUCLEIC ACIDS RES, V9, P2989

[12]

HOTTE B, 1990, J BACTERIOL, V172, P2804

[13] A PLANT-INDUCIBLE GENE OF XANTHOMONAS-CAMPESTRIS PV CAMPESTRIS ENCODES AN EXOCELLULAR COMPONENT REQUIRED FOR GROWTH IN THE HOST AND HYPERSENSITIVITY ON NONHOSTS [J].

KAMOUN, S ;

KADO, CI .

JOURNAL OF BACTERIOLOGY, 1990, 172 (09) :5165-5172

[14] THE OPSX LOCUS OF XANTHOMONAS-CAMPESTRIS AFFECTS HOST-RANGE AND BIOSYNTHESIS OF LIPOPOLYSACCHARIDE AND EXTRACELLULAR POLYSACCHARIDE [J].

KINGSLEY, MT ;

GABRIEL, DW ;

MARLOW, GC ;

ROBERTS, PD .

JOURNAL OF BACTERIOLOGY, 1993, 175 (18) :5839-5850

[15] SENSITIVE SILVER-STAINING DETECTION OF BACTERIAL LIPOPOLYSACCHARIDES IN POLYACRYLAMIDE GELS [J].

KITTELBERGER, R ;

HILBINK, F .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1993, 26 (01) :81-86

[16]

KOPLIN R, 1992, J BACTERIOL, V174, P191

[17]

KOPLIN R, 1993, J BACTERIOL, V175, P7786

[18] EXOPOLYSACCHARIDES IN PLANT-BACTERIAL INTERACTIONS [J].

LEIGH, JA ;

COPLIN, DL .

ANNUAL REVIEW OF MICROBIOLOGY, 1992, 46 :307-346

[19] INCREASED RESOLUTION OF LIPOPOLYSACCHARIDES AND LIPOOLIGOSACCHARIDES UTILIZING TRICINE-SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS [J].

LESSE, AJ ;

CAMPAGNARI, AA ;

BITTNER, WE ;

APICELLA, MA .

JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 126 (01) :109-117

[20]

LIN TW, 1992, J BIOL CHEM, V267, P975

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