Ubiquitin binding proteins protect ubiquitin conjugates from disassembly

被引：46

作者：

Hartmann-Petersen, R ^{[1
]}

Hendil, KB ^{[1
]}

Gordon, C ^{[1
]}

机构：

[1] Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland

来源：

FEBS LETTERS | 2003年 / 535卷 / 1-3期

关键词：

ubiquitin; proteasome; deubiquitinating enzyme; ubiquitin-C-terminal hydrolase; ubiquitin pathway associated domain; ubiquitin interaction motif;

D O I：

10.1016/S0014-5793(02)03874-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As a step in their turnover proteins in eukaryotic cells are coupled to a small protein, ubiquitin, before they are recognised by 26S proteasomes and degraded. However, cells also contain many deubiquitinating enzymes, which can rescue proteins by cleaving off the ubiquitin chains. Here we report that three ubiquitin binding proteins, Rhp23, Dph1 and Pus1, from fission yeast can protect multiubiquitin conjugates against deubiquitination. This protection depends on the ubiquitin binding domains and may promote degradation of ubiquitinated proteins. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：77 / 81

页数：5

共 36 条

[1]

Baker RT, 2000, MOL B INT U, V12, P236

[2] A novel active site-directed probe specific for deubiquitylating enzymes reveals proteasome association of USP14 [J].

Borodovsky, A ;

Kessler, BM ;

Casagrande, R ;

Overkleeft, HS ;

Wilkinson, KD ;

Ploegh, HL .

EMBO JOURNAL, 2001, 20 (18) :5187-5196

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] Rad23 promotes the targeting of proteolytic substrates to the proteasome [J].

Chen, L ;

Madura, K .

MOLECULAR AND CELLULAR BIOLOGY, 2002, 22 (13) :4902-4913

[5] Dosage suppressors of pds1 implicate ubiquitin-associated domains in checkpoint control [J].

Clarke, DJ ;

Mondesert, G ;

Segal, M ;

Bertolaet, BL ;

Jensen, S ;

Wolff, M ;

Henze, M ;

Reed, SI .

MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (06) :1997-2007

[6]

DEVERAUX Q, 1994, J BIOL CHEM, V269, P7059

[7] Structure of a human DNA repair protein UBA domain that interacts with HIV-1 Vpr [J].

Dieckmann, T ;

Withers-Ward, ES ;

Jarosinski, MA ;

Liu, CF ;

Chen, ISY ;

Feigon, J .

NATURE STRUCTURAL BIOLOGY, 1998, 5 (12) :1042-1047

[8] Involvement of rhp23, a Schizosaccharomyces pombe homolog of the human HHR23A and Saccharomyces cerevisiae RAD23 nucleotide excision repair genes, in cell cycle control and protein ubiquitination [J].

Elder, RT ;

Song, XQ ;

Chen, MZ ;

Hopkins, KM ;

Lieberman, HB ;

Zhao, YQ .

NUCLEIC ACIDS RESEARCH, 2002, 30 (02) :581-591

[9] Proteasome subunit Rpn1 binds ubiquitin-like protein domains [J].

Elsasser, S ;

Gali, RR ;

Schwickart, M ;

Larsen, CN ;

Leggett, DS ;

Müller, B ;

Feng, MT ;

Tübing, F ;

Dittmar, GAG ;

Finley, D .

NATURE CELL BIOLOGY, 2002, 4 (09) :725-730

[10] Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome [J].

Funakoshi, M ;

Sasaki, T ;

Nishimoto, T ;

Kobayashi, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (02) :745-750

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