Rapid resolution of carbohydrate isomers by electrospray ionization ambient pressure ion mobility spectrometry-time-of-flight mass spectrometry (ESI-APIMS-TOFMS)

被引:147
作者
Dwivedi, Prabha
Bendiak, Brad
Clowers, Brian H.
Hill, Herbert H., Jr. [1 ]
机构
[1] Washington State Univ, Dept Chem, Pullman, WA 99164 USA
[2] Washington State Univ, Ctr Multiphase Environm Res, Pullman, WA 99164 USA
[3] Univ Colorado, Hlth Sci Ctr, Cell & Dev Biol & Biomol Struct Program, Aurora, CO USA
关键词
D O I
10.1016/j.jasms.2007.04.007
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Carbohydrates are an extremely complex group of isomeric molecules that have been difficult to analyze in the gas phase by mass spectrometry because (1) precursor ions and product ions to successive stages of MSn are frequently mixtures of isomers, and (2) detailed information about the anomeric configuration and location of specific stereochemical variants of monosaccharides within larger molecules has not been possible to obtain in a general way. Herein, it is demonstrated that gas-phase analyses by direct combination of electrospray ionization, ambient pressure ion mobility spectrometry, and time-of-flight mass spectrometry (ESI-APIMS-TOFMS) provides sufficient resolution to separate different anomeric methyl glycosides and to separate different stereoisomeric methyl glycosides having the same anomeric configuration. Reducing sugars were typically resolved into more than one peak, which might represent separation of cyclic species having different anomeric configurations and/or ring forms. The extent of separation, both with methyl glycosides and reducing sugars, was significantly affected by the nature of the drift gas and by the nature of an adducting metal ion or ion complex. The study demonstrated that ESI-APIMS-TOFMS is a rapid and effective analytical technique for the separation of isomeric methyl glycosides and simple sugars, and can be used to differentiate glycosides having different anomeric configurations.
引用
收藏
页码:1163 / 1175
页数:13
相关论文
共 37 条
[1]   Using different drift cases to change separation factors (α) in ion mobility spectrometry [J].
Asbury, GR ;
Hill, HH .
ANALYTICAL CHEMISTRY, 2000, 72 (03) :580-584
[2]  
Asbury GR, 2000, J MICROCOLUMN SEP, V12, P172, DOI 10.1002/(SICI)1520-667X(2000)12:3<172::AID-MCS7>3.0.CO
[3]  
2-W
[4]   Congruent strategies for carbohydrate sequencing.: 1.: Mining structural details by MSn [J].
Ashline, D ;
Singh, S ;
Hanneman, A ;
Reinhold, V .
ANALYTICAL CHEMISTRY, 2005, 77 (19) :6250-6262
[5]   Effects of drift-gas polarizability on glycine peptides in ion mobility spectrometry [J].
Beegle, LW ;
Kanik, I ;
Matz, L ;
Hill, HH .
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2002, 216 (03) :257-268
[6]   Sequential removal of monosaccharides from the reducing end of oligosaccharides part IV - End-group determination of oligosaccharides: a gas chromatography-mass spectrometry/mass spectrometry method for distinguishing between all D-aldohexoses and D-ketohexoses [J].
Bendiak, B ;
Fang, TT .
CARBOHYDRATE RESEARCH, 2000, 327 (04) :463-481
[7]  
BENDIAK B, 1998, EUR J BIOCHEM, V258, P603
[8]   Separation of sodiated isobaric disaccharides and trisaccharides using electrospray ionization-atmospheric pressure ion mobility-time of flight mass spectrometry [J].
Clowers, BH ;
Dwivedi, P ;
Steiner, WE ;
Hill, HH ;
Bendiak, B .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2005, 16 (05) :660-669
[9]  
COHEN MJ, 1971, Patent No. 3699333
[10]   Developments in ion mobility spectrometry-mass spectrometry [J].
Collins, DC ;
Lee, ML .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2002, 372 (01) :66-73