The regulation of photosynthetic electron transport during nutrient deprivation in Chlamydomonas reinhardtii

被引:351
作者
Wykoff, DD
Davies, JP
Melis, A
Grossman, AR
机构
[1] Carnegie Inst Washington, Dept Plant Biol, Stanford, CA 94305 USA
[2] Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA
[3] Univ Calif Berkeley, Dept Plant Biol, Berkeley, CA 94720 USA
关键词
D O I
10.1104/pp.117.1.129
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The light-saturated rate of photosynthetic O-2 evolution in Chlamydomonas reinhardtii declined by approximately 75% on a per-cell basis after 4 d of P starvation or 1 d of S starvation. Quantitation of the partial reactions of photosynthetic electron transport demonstrated that the light-saturated rate of photosystem (PS) I activity was unaffected by P or S limitation, whereas light-saturated PSII activity was reduced by more than 50%. This decline in PSII activity correlated with a decline in both the maximal quantum efficiency of PSII and the accumulation of the secondary quinone electron acceptor of PSII nonreducing centers (PSII centers capable of performing a charge separation but unable to reduce the plastoquinone pool). In addition to a decline in the light-saturated rate of O-2 evolution, there was reduced efficiency of excitation energy transfer to the reaction centers of PSII (because of dissipation of absorbed tight energy as heat and because of a transition to state 2). These findings establish a common suite of alterations in photosynthetic electron transport that results in decreased linear electron flow when C. reinhardtii is limited for either P or S. It was interesting that the decline in the maximum quantum efficiency of PSII and the accumulation of the secondary quinone electron acceptor of PSII nonreducing centers were regulated specifically during S-limited growth by the Sad gene product, which was previously shown to be critical for the acclimation of C. reinhardtii to S limitation (J.P. Davies, F.H. Yildiz, and A.R. Grossman [1996] EMBO J 15:2150-2159).
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页码:129 / 139
页数:11
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