Cloning and characterization of a novel human chemokine receptor

被引:40
作者
Fan, P [1 ]
Kyaw, H [1 ]
Su, K [1 ]
Zeng, ZZ [1 ]
Augustus, M [1 ]
Carter, KC [1 ]
Li, Y [1 ]
机构
[1] Human Genome Sci Inc, Rockville, MD 20850 USA
关键词
D O I
10.1006/bbrc.1997.7981
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The present study reports the identification of a human gene, HCR, which encodes a novel human chemokine receptor. The partial sequence of the HCR gene was first found in a human neutrophil cDNA Library. With the use of an expressed sequence tag (EST) probe from the neutrophil library, the full length HCR cDNA was isolated. The open reading frame of HCR cDNA predicts a protein of 345 amino acids with seven transmembrane domain topography. The HCR gene exhibits good homology to human MIP-1a receptor with 43.1% amino acid identity and 64.4% amino acid similarity and also shows considerable sequence homology to other human chemokine receptors such as the MCP-3 receptor, MCP-5 receptor, and MCP-1 receptor. Northern blot analysis suggests that HCR gene is expressed abundantly in immunal tissues such as spleen, fetal liver, lymph node, and bone marrow. Strong expression was also found in human lung and heart. A chromosome mapping study indicated that HCR gene is positioned within human chromosome band Xq13. Our result suggests that HCR gene is a novel putative chemokine receptor. (C) 1998 Academic Press.
引用
收藏
页码:264 / 268
页数:5
相关论文
共 22 条
[1]   SEQUENCE IDENTIFICATION OF 2,375 HUMAN BRAIN GENES [J].
ADAMS, MD ;
DUBNICK, M ;
KERLAVAGE, AR ;
MORENO, R ;
KELLEY, JM ;
UTTERBACK, TR ;
NAGLE, JW ;
FIELDS, C ;
VENTER, JC .
NATURE, 1992, 355 (6361) :632-634
[2]  
ADAMS MD, 1995, NATURE, V377, P3
[3]   COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT [J].
ADAMS, MD ;
KELLEY, JM ;
GOCAYNE, JD ;
DUBNICK, M ;
POLYMEROPOULOS, MH ;
XIAO, H ;
MERRIL, CR ;
WU, A ;
OLDE, B ;
MORENO, RF ;
KERLAVAGE, AR ;
MCCOMBIE, WR ;
VENTER, JC .
SCIENCE, 1991, 252 (5013) :1651-1656
[4]   MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF 2 MONOCYTE CHEMOATTRACTANT PROTEIN-1 RECEPTORS REVEALS ALTERNATIVE SPLICING OF THE CARBOXYL-TERMINAL TAILS [J].
CHARO, IF ;
MYERS, SJ ;
HERMAN, A ;
FRANCI, C ;
CONNOLLY, AJ ;
COUGHLIN, SR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (07) :2752-2756
[5]   CLONING AND FUNCTIONAL EXPRESSION OF A HUMAN EOSINOPHIL CC-CHEMOKINE RECEPTOR [J].
COMBADIERE, C ;
AHUJA, SK ;
MURPHY, PM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (28) :16491-16494
[6]   Identification of a major co-receptor for primary isolates of HIV-1 [J].
Deng, HK ;
Liu, R ;
Ellmeier, W ;
Choe, S ;
Unutmaz, D ;
Burkhart, M ;
DiMarzio, P ;
Marmon, S ;
Sutton, RE ;
Hill, CM ;
Davis, CB ;
Peiper, SC ;
Schall, TJ ;
Littman, DR ;
Landau, NR .
NATURE, 1996, 381 (6584) :661-666
[7]   HIV-1 entry into CD4(+) cells is mediated by the chemokine receptor CC-CKR-5 [J].
Dragic, T ;
Litwin, V ;
Allaway, GP ;
Martin, SR ;
Huang, YX ;
Nagashima, KA ;
Cayanan, C ;
Maddon, PJ ;
Koup, RA ;
Moore, JP ;
Paxton, WA .
NATURE, 1996, 381 (6584) :667-673
[8]   CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia [J].
He, JL ;
Chen, YZ ;
Farzan, M ;
Choe, HY ;
Ohagen, A ;
Gartner, S ;
Busciglio, J ;
Yang, XY ;
Hofmann, W ;
Newman, W ;
Mackay, CR ;
Sodroski, J ;
Gabuzda, D .
NATURE, 1997, 385 (6617) :645-649
[9]   Chemokines: Progress toward identifying molecular targets for therapeutic agents [J].
Howard, OMZ ;
BenBaruch, A ;
Oppenheim, JJ .
TRENDS IN BIOTECHNOLOGY, 1996, 14 (02) :46-51
[10]   A SIMPLE, RAPID TECHNIQUE FOR PRECISE MAPPING OF MULTIPLE SEQUENCES IN 2 COLORS USING A SINGLE OPTICAL FILTER SET [J].
JOHNSON, CV ;
MCNEIL, JA ;
CARTER, KC ;
LAWRENCE, JB .
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING, 1991, 8 (02) :75-76