Control of ftsZ expression, cell division, and glutamine metabolism in Luria-Bertani medium by the alarmone ppGpp in Escherichia coli

被引:46
作者
Powell, BS [1 ]
Court, DL [1 ]
机构
[1] NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Frederick, MD 21702 USA
关键词
D O I
10.1128/JB.180.5.1053-1062.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Inactivation of transcription factor sigma(54), encoded by rpoN (glnF), restores high-temperature growth in Luria-Bertani (LB) medium to strains containing the heat-sensitive cell division mutation ftsZ84. Mutational defects in three other genes involved in general nitrogen control (glnD, glnG, and glnL) also suppress lethal filamentation. Since addition of glutamine to LB medium fully blocks suppression by each mutation, the underlying cause of suppression likely derives from a stringent response to the limitation of glutamine. This model is supported by several observations, The glnL mutation requires RelA-directed synthesis of the nutrient alarmone ppGpp to suppress filamentation. Artificially elevated levels of ppGpp suppress ftsZ84, as do RNA polymerase mutations that reproduce global effects of the ppGpp-induced state. Both the glnF null mutation and an elevated copy number of the relA gene similarly affect transcription from the upstream (pQ) promoters of the ftsQAZ operon, and both of these genetic conditions increase the steady-state level of the FtsZ84 protein, Physiological suppression of ftsZ84 by a high salt concentration was also shown to involve RelA. Additionally, we found that the growth of a glnF or glnD strain on LB medium depends on RelA or supplemental glutamine in the absence of RelA function, These data expand the roles for ppGpp in the regulation of glutamine metabolism and the expression of FtsZ during cell division.
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页码:1053 / 1062
页数:10
相关论文
共 72 条
[31]   DETERMINATION OF TRANSCRIPTIONAL UNITS AND GENE-PRODUCTS FROM THE FTSA REGION OF ESCHERICHIA-COLI [J].
LUTKENHAUS, JF ;
WU, HC .
JOURNAL OF BACTERIOLOGY, 1980, 143 (03) :1281-1288
[32]   GENETIC-CONTROL OF NITROGEN ASSIMILATION IN BACTERIA [J].
MAGASANIK, B .
ANNUAL REVIEW OF GENETICS, 1982, 16 :135-168
[33]   REGULATION OF TRANSCRIPTION OF THE GLNALG OPERON OF ESCHERICHIA-COLI BY PROTEIN-PHOSPHORYLATION [J].
MAGASANIK, B .
BIOCHIMIE, 1989, 71 (9-10) :1005-1012
[34]   THE REGULATION OF NITROGEN-UTILIZATION IN ENTERIC BACTERIA [J].
MAGASANIK, B .
JOURNAL OF CELLULAR BIOCHEMISTRY, 1993, 51 (01) :34-40
[35]  
Miller J.H., 1992, SHORT COURSE BACTERI, V1
[36]   GUANINE NUCLEOTIDE-DEPENDENT ASSEMBLY OF FTSZ INTO FILAMENTS [J].
MUKHERJEE, A ;
LUTKENHAUS, J .
JOURNAL OF BACTERIOLOGY, 1994, 176 (09) :2754-2758
[37]   ROLE OF GUANOSINE TETRAPHOSPHATE IN GENE-EXPRESSION AND THE SURVIVAL OF GLUCOSE OR SERYL-TRANSFER-RNA STARVED CELLS OF ESCHERICHIA-COLI K12 [J].
NYSTROM, T .
MOLECULAR AND GENERAL GENETICS, 1994, 245 (03) :355-362
[38]   INTERRELATED EFFECTS OF DNA SUPERCOILING, PPGPP, AND LOW SALT ON MELTING WITHIN THE ESCHERICHIA-COLI RIBOSOMAL-RNA RRNBP1 PROMOTER [J].
OHLSEN, KL ;
GRALLA, JD .
MOLECULAR MICROBIOLOGY, 1992, 6 (16) :2243-2251
[39]   Dependency of Escherichia coli cell-division size, and independency of nucleoid segregation on the mode and level of ftsZ expression [J].
Palacios, P ;
Vicente, M ;
Sanchez, M .
MOLECULAR MICROBIOLOGY, 1996, 20 (05) :1093-1098
[40]   CELL-DIVISION CONTROL IN ESCHERICHIA-COLI K-12 - SOME PROPERTIES OF THE FTSZ84 MUTATION AND SUPPRESSION OF THIS MUTATION BY THE PRODUCT OF A NEWLY IDENTIFIED GENE [J].
PHOENIX, P ;
DRAPEAU, GR .
JOURNAL OF BACTERIOLOGY, 1988, 170 (09) :4338-4342