Detecting SNPs using a synthetic nanopore

被引：108

作者：

Zhao, Q. ^{[1
]}

Sigalov, G. ^{[1
]}

Dimitrov, V. ^{[1
]}

Dorvel, B. ^{[1
]}

Mirsaidov, U. ^{[1
]}

Sligar, S. ^{[1
]}

Aksimentiev, A. ^{[1
]}

Timp, G. ^{[1
]}

机构：

[1] Univ Illinois, Beckman Inst, Urbana, IL 61801 USA

来源：

NANO LETTERS | 2007年 / 7卷 / 06期

关键词：

D O I：

10.1021/nl070668c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have discovered a voltage threshold for permeation through a synthetic nanopore of dsDNA bound to a restriction enzyme that depends on the sequence. Molecular dynamic simulations reveal that the threshold is associated with a nanonewton force required to rupture the DNA-protein complex. A single mutation in the recognition site for the restriction enzyme, i.e., a single nucleotide polymorphism (SNP), can easily be detected as a change in the threshold voltage. Consequently, by measuring the threshold voltage in a synthetic nanopore, it may be possible to discriminate between two variants of the same gene (alleles) that differ in one base.

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页码：1680 / 1685

页数：6

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