Rapid identification of heterotrophic, thermophilic, spore-forming bacteria isolated from hot composts

被引:64
作者
Blanc, M
Marilley, L
Beffa, T
Aragno, M
机构
[1] Laboratoire de Microbiologie, Université de Neuchâtel
[2] Laboratoire de Microbiologie, Université de Neuchâtel, CH-2007 Neuchâtel
来源
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY | 1997年 / 47卷 / 04期
关键词
D O I
10.1099/00207713-47-4-1246
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The restriction enzyme profiles of 16S ribosomalk DNAs (rDNAs) amplified qv PCR from thermophilic heterotrophic bacterial strains isolated from composts were compared with those of reference strains. This allowed us to assign ail but 1 of 16 strains to four different Bacillus species (namely, Bacillus stearothermophilus, Bacillus pallidus, Bacillus thermoglucosidasius, and ''Bacillus thermodentrificans''). This study showed that PCR restriction analysis of 16S rDNA contributes to rapid and reliable identification of newly isolated strains belonging to recognized species.
引用
收藏
页码:1246 / 1248
页数:3
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