A strategy for functional proteomic analysis of glycosidase activity from cell lysates

被引：115

作者：

Vocadlo, DJ

Bertozzi, CR

机构：

[1] Univ Calif Berkeley, Ctr New Direct Organ Synth, Howard Hughes Med Inst, Dept Chem, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[3] Simon Fraser Univ, Dept Chem, Burnaby, BC V5A 1A6, Canada

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 40期

关键词：

carbohydrates; glycosiclases; mechanism-based inactivators; proteomics; Staudinger ligation;

D O I：

10.1002/anie.200454235

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A multipurpose flag: The inactivator 1 covalently labels the catalytic nucleophiles of retaining β-glycosidases to form species 2. The small azide group allows labeling of enzymes with sterically congested active sites. Staudinger ligation of 2 with phosphine-FLAG yields adduct 3, which can be used to detect and profile retaining β-glycosidase activities in complex mixtures.

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收藏

页码：5338 / 5342

页数：5

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