Zebrafish relatively relaxed mutants have a ryanodine receptor defect, show slow swimming and provide a model of multi-minicore disease

被引:97
作者
Hirata, Hiromi [1 ]
Watanabe, Takaki
Hatakeyama, Jun
Sprague, Shawn M.
Saint-Amant, Louis
Nagashima, Ayako
Cui, Wilson W.
Zhou, Weibin
Kuwada, John Y.
机构
[1] Kumamoto Univ, Inst Mol Embryol & Genet, Kumamoto 860, Japan
[2] Nagoya Univ, Grad Sch Sci, Nagoya, Aichi 464, Japan
[3] Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA
[4] Univ Montreal, Dept Pathol & Biol Cellulaire, Montreal, PQ H3T 1J4, Canada
来源
DEVELOPMENT | 2007年 / 134卷 / 15期
关键词
zebrafish; ryanodine receptor; muscle; calcium; multi-minicore disease;
D O I
10.1242/dev.004531
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Wild-type zebrafish embryos swim away in response to tactile stimulation. By contrast, relatively relaxed mutants swim slowly due to weak contractions of trunk muscles. Electrophysiological recordings from muscle showed that output from the CNS was normal in mutants, suggesting a defect in the muscle. Calcium imaging revealed that Ca2+ transients were reduced in mutant fast muscle. Immunostaining demonstrated that ryanodine and dihydropyridine receptors, which are responsible for Ca2+ release following membrane depolarization, were severely reduced at transverse-tubule/sarcoplasmic reticulum junctions in mutant fast muscle. Thus, slow swimming is caused by weak muscle contractions due to impaired excitation-contraction coupling. Indeed, most of the ryanodine receptor 1b (ryr1b) mRNA in mutants carried a nonsense mutation that was generated by aberrant splicing due to a DNA insertion in an intron of the ryr1b gene, leading to a hypomorphic condition in relatively relaxed mutants. RYR1 mutations in humans lead to a congenital myopathy, multi-minicore disease (MmD), which is defined by amorphous cores in muscle. Electron micrographs showed minicore structures in mutant fast muscles. Furthermore, following the introduction of antisense morpholino oligonucleotides that restored the normal splicing of ryr1b, swimming was recovered in mutants. These findings suggest that zebrafish relatively relaxed mutants may be useful for understanding the development and physiology of MmD.
引用
收藏
页码:2771 / 2781
页数:11
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