Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes

被引：51

作者：

Glossop, John R. ^{[1
,2
]}

Emes, Richard D. ^{[3
,4
]}

Nixon, Nicola B. ^{[2
]}

Packham, Jon C. ^{[2
]}

Fryer, Anthony A. ^{[1
]}

Mattey, Derek L. ^{[1
,2
]}

Farrell, William E. ^{[1
]}

机构：

[1] Keele Univ, Guy Hilton Res Ctr, Inst Sci & Technol Med, Thornburrow Dr, Stoke On Trent ST4 7QB, Staffs, England

[2] Haywood Hosp, Haywood Rheumatol Ctr, High Lane, Stoke On Trent ST6 7AG, Staffs, England

[3] Univ Nottingham, Sch Vet Med & Sci, Sutton Bonington Campus, Loughborough LE12 5RD, Leics, England

[4] Univ Nottingham, Adv Data Anal Ctr, Sutton Bonington Campus, Loughborough LE12 5RD, Leics, England

来源：

EPIGENOMICS | 2016年 / 8卷 / 02期

关键词：

B lymphocyte; CpG; DNA methylation; early rheumatoid arthritis; genome-wide; Illumina 450K array; T lymphocyte; treatment naive; SINGLE CPG SITE; METHYLATION PATTERNS; UPSTREAM ENHANCER; CELL-FUNCTION; FOXP3; HYPOMETHYLATION; IDENTIFICATION; DEMETHYLATION; METHOTREXATE; DISCOVERY;

D O I：

10.2217/epi.15.103

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学];

摘要：

Aim: Although aberrant DNA methylation has been described in rheumatoid arthritis (RA), no studies have interrogated this epigenetic modification in early disease. Following recent investigations of T and B lymphocytes in established disease, we now characterize in these cell populations genome-wide DNA methylation in treatment-naive patients with early RA. Patients & methods: HumanMethylation450 BeadChips were used to examine genome-wide DNA methylation in lymphocyte populations from 23 early RA patients and 11 healthy individuals. Results: Approximately 2000 CpGs in each cell type were differentially methylated in early RA. Clustering analysis identified a novel methylation signature in each cell type (150 sites in T lymphocytes, 113 sites in B lymphocytes) that clustered all patients separately from controls. A subset of sites differentially methylated in early RA displayed similar changes in established disease. Conclusion: Treatment-naive early RA patients display novel disease-specific DNA methylation aberrations, supporting a potential role for these changes in the development of RA.

引用

页码：209 / 224

页数：16

共 53 条

[1]

2010 Rheumatoid Arthritis Classification Criteria An American College of Rheumatology/European League Against Rheumatism Collaborative Initiative [J].