High-throughput screening of enzyme libraries

被引:101
作者
Olsen, M [1 ]
Iverson, B
Georgiou, G
机构
[1] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA
[2] Univ Texas, Dept Chem Engn, Austin, TX 78712 USA
关键词
D O I
10.1016/S0958-1669(00)00108-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Directed evolution is becoming a widely used technique for modifying or enhancing protein performance. Ultimately, the success of directed protein evolution experiments hinges on the efficiency of the methods used to screen libraries for mutants with properties of interest. Although there is still a paucity of general methods for enzyme library screening, in recent years a number of promising strategies have emerged and are increasingly being used to explore challenging issues in protein engineering.
引用
收藏
页码:331 / 337
页数:7
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