Recombinant expression of lymphocytic choriomeningitis virus strain WE glycoproteins: a single amino acid makes the difference

被引：33

作者：

Beyer, WR ^{[1
]}

Miletic, H ^{[1
]}

Ostertag, W ^{[1
]}

von Laer, D ^{[1
]}

机构：

[1] Univ Hamburg, Heinrich Pette Inst Expt Virol & Immunol, D-20251 Hamburg, Germany

来源：

JOURNAL OF VIROLOGY | 2001年 / 75卷 / 02期

关键词：

D O I：

10.1128/JVI.75.2.1061-1064.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cytoplasmic vector systems are generally used for expression of lymphocytic choriomeningitis virus (LCMV) proteins. However, we achieved high levels of cell surface glycoproteins using a standard nuclear expression plasmid. Expression was independent of other LCMV proteins but was blocked by a missense mutation within the original LCMV(WE) glycoprotein cDNA.

引用

页码：1061 / 1064

页数：4

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