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A hydrogel-based stem cell delivery system to treat retinal degenerative diseases

被引：181
作者：
Ballios, Brian G. [1 ]
Cooke, Michael J. [2 ]
van der Kooy, Derek [1 ,3 ]
Shoichet, Molly S. [1 ,2 ,4 ]
机构：
[1] Univ Toronto, Inst Med Sci, Toronto, ON M5S 1A8, Canada
[2] Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3E5, Canada
[3] Univ Toronto, Dept Med Genet, Toronto, ON M5S 1A8, Canada
[4] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5S 3G9, Canada
来源：
BIOMATERIALS | 2010年 / 31卷 / 09期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Hyaluronan; Hydrogel; Retina; Stem cell; Cell viability; Cell spreading; INJURED SPINAL-CORD; PROGENITOR CELLS; DRUG-DELIVERY; INTERPHOTORECEPTOR MATRIX; DIABETIC-RETINOPATHY; MACULAR DEGENERATION; LOCALIZED DELIVERY; IN-VIVO; DIFFERENTIATION; INTEGRATION;
D O I：
10.1016/j.biomaterials.2009.12.004
中图分类号：
R318 [生物医学工程];
学科分类号：
100103 [病原生物学];
摘要：
Regenerative strategies for retinal degenerative diseases are limited by poor Cellular survival distribution and Integration after transplantation to the sub-retinal space To overcome this limitations a stem cell delivery system was developed, taking advantage of the minimally-invasive, injectable and biodegradable properties of a blend Of hyaluronan and methylcellulose (HAMC) The physical and biological properties of this unique HAMC formulation were studied HAMC supported retinal stern-progenitor cell (RSPC) survival and proliferation in vitro The blend Was a VISCOUS Solution, exhibiting properties ideal for delivery to the sub-retinal space In vivo transplantation Studies in mice were carried out to investigate both the biodegradability of HAMC in the sub-retinal space over 7 clays and the potential of HAMC as a cell delivery vehicle RSPCs delivered in HAMC were more evenly distributed in the sub-retinal Space than those delivered in traditional saline Solutions. Suggesting that HAMC is a promising vehicle for Cellular delivery to the degenerating retina overcoming previously reported barriers to tissue integration in the retina such as cellular aggregation and non-contiguous distribution (C) 2009 Elsevier Ltd All rights reserved
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收藏
页码：2555 / 2564
页数：10
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