Efficient activation of nucleoside phosphoramidites with 4,5-dicyanoimidazole during oligonucleotide synthesis

被引：110

作者：

Vargeese, C ^{[1
]}

Carter, J ^{[1
]}

Yegge, J ^{[1
]}

Krivjansky, S ^{[1
]}

Settle, A ^{[1
]}

Kropp, E ^{[1
]}

Peterson, K ^{[1
]}

Pieken, W ^{[1
]}

机构：

[1] NeXstar Technol Prod, Boulder, CO 80301 USA

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 04期

关键词：

D O I：

10.1093/nar/26.4.1046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new activator for the coupling of phosphoramidites to the 5'-hydroxyl group during oligonucleotide synthesis is introduced. The observed time to complete coupling is twice as fast with 4,5-dicyanoimidazole (DCI) as the activator, compared with 1H-tetrazole. The effectiveness of DCI is thought to be based on its nucleophilicity. DCI is soluble in acetonitrile up to 1.1 M at room temperature and can be used as the sole coupling activator during routine automated solid phase synthesis of oligonucleotides. The addition of 0.1 M N-methylimidazole to 0.45 M 1H-tetrazole also results in higher product yields during oligonucleotide synthesis than observed with 1H-tetrazole alone.

引用

页码：1046 / 1050

页数：5

共 15 条

[1]

AGRAWAL S, 1996, TRENDS BIOCH TECHNOL, V14, P375

[2] DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS [J].