Enhancement of cell survival by stromal cell-derived factor-1/CXCL12 involves activation of CREB and induction of Mcl-1 and c-Fos in factor-dependent human cell line MO7e

被引：24

作者：

Joo, EK

Broxmeyer, HE

Kwon, HJ

Kang, HB

Kim, JS

Lim, JS

Choe, YK

Choe, IS

Myung, PK

Lee, Y

机构：

[1] Chungbuk Natl Univ, Dept Biochem, Biotechnol Res Inst, Cheongju 361763, Chungbuk, South Korea

[2] Indiana Univ, Sch Med, Dept Microbiol & Immunol, Indianapolis, IN 46202 USA

[3] Indiana Univ, Sch Med, Walther Oncol Ctr, Indianapolis, IN 46202 USA

[4] Yonsei Univ, Inst Life Sci & Biotechnol, Seoul 120749, South Korea

[5] KRIBB, Cell Biol Lab, Taejon 305600, South Korea

[6] Chungnam Natl Univ, Coll Pharm, Taejon 305764, South Korea

来源：

STEM CELLS AND DEVELOPMENT | 2004年 / 13卷 / 05期

关键词：

D O I：

10.1089/1547328042417318

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Stromal cell-derived factor-1 (SDF-1/CXCL12) enhances the survival of hematopoietic stem and progenitor cells in synergy with other cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF), steel factor, and thrombopoietin (TPO), and both the PI3K/Akt and MAPK pathways have been linked to this survival. To further evaluate intracellular signaling involved in SDF-1/CXCL12 survival effects, we investigated modulation of downstream signaling molecules. The synergistic survival enhancement of SDF-1/CXCL12 plus other cytokines were directly linked to enhanced phosphorylation of p70/85S6K and cAMP responsive element binding protein ( CREB), as well as enhanced induction of the Bcl-2 family member Mcl-1. Most prominently, c-Fos, a component of AP1 transcription factor, was synergistically induced by SDF-1/CXCL12 plus other cytokines. These results suggest that SDF-1/CXCL12 enhanced cell survival in synergy with other cytokines involves activation of CREB and induction of Mcl-1 and c-Fos.

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收藏

页码：563 / 570

页数：8

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