Phenolic extractives from the bark of Pinus sylvestris L. and their effects on inflammatory mediators nitric oxide and prostaglandin E2

被引：88

作者：

Karonen, M

Hämäläinen, M

Nieminen, R

Klika, KD

Loponen, J

Ovcharenko, VV

Moilanen, E

Pihlaja, K

机构：

[1] Univ Turku, Dept Chem, Environm Chem & Struct Chem Grp, FIN-20014 Turku, Finland

[2] Univ Tampere, Sch Med, Immunopharmacol Res Grp, FIN-33014 Tampere, Finland

[3] Tampere Univ Hosp, FIN-33521 Tampere, Finland

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2004年 / 52卷 / 25期

关键词：

bark; HPLC-DAD; HPLC-ESI-MS; inflammation; macrophages; nitric oxide; nitric oxide synthase; phenolic compounds; Pinus sylvestris L;

D O I：

10.1021/jf048948q

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The anti-inflammatory properties of phenolic pine (Pinus sylvestris L.) bark extract were studied. The pine bark extract was fractionated by liquid-liquid extractions and semipreparative high-performance liquid chromatography to reveal the most potent constituents. The phenolic compositions of three pine bark samples obtained, a crude extract, a chloroform fraction, and a semipreparative fraction, were analyzed using high-performance liquid chromatography with UV diode array detection and/or electrospray ionization mass spectrometry. In addition, eight compounds were isolated and identified by NMR and MS techniques. In total 28 phenolic compounds were identified. The effects of the three pine bark samples on the synthesis of two proinflammatory mediators, nitric oxide and prostaglandin E-2, were measured. It was shown that pine bark contains compounds that inhibit the production of these proinflammatory mediators.

引用

页码：7532 / 7540

页数：9

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