Glycosidase mechanisms

被引：438

作者：

Rye, CS ^{[1
]}

Withers, SG ^{[1
]}

机构：

[1] Univ British Columbia, Vancouver, BC V6T 1Z1, Canada

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2000年 / 4卷 / 05期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1016/S1367-5931(00)00135-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insights into glycosidase mechanisms have come from X-ray crystallographic studies on complexes with substrate analogs and inhibitors, representing all the intermediate species along the reaction coordinate. Site-directed mutagenesis continues to play a significant role in understanding mechanisms, but is also proving important in generating glycosidases of modified mechanism or specificity.

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页码：573 / 580

页数：8

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