Transcriptional activating regions target attached substrates to a cyclin-dependent kinase

被引：13

作者：

Ansari, AZ

Ogirala, A

Ptashne, M

机构：

[1] Sloan Kettering Inst, Program Mol Biol, New York, NY 10021 USA

[2] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

[3] Univ Wisconsin, Genome Ctr, Madison, WI 53706 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 07期

关键词：

kinase-substrate targeting; proteolysis; Srb; 10/11; transcriptional regulation;

D O I：

10.1073/pnas.0409671102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The yeast cyclin-dependent kinase Srb10 phosphorylates various transcriptional activators as they activate transcription, and acidic transcriptional activating domains found on several activators directly bind Srb10. Here we show that the interaction between Srb10 (with its associated cyclin Srb11) and each of several different activating regions, in vitro, leads to the phosphorylation of peptide sequences attached to but outside of the activating regions themselves. In some cases, residues within the activating regions are also phosphorylated. The results define a mechanism by which a kinase is recruited to alternate substrates with diverse physiological consequences.

引用

收藏

页码：2346 / 2349

页数：4

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