Application of high-resolution DNA melting for genotyping and variant scanning of diploid and autotetraploid potato

被引：55

作者：

De Koeyer, David ^{[1
]}

Douglass, Katheryn ^{[1
]}

Murphy, Agnes ^{[1
]}

Whitney, Sean ^{[1
]}

Nolan, Lana ^{[1
,2
]}

Song, Yong ^{[1
,3
]}

De Jong, Walter ^{[4
]}

机构：

[1] Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB E3B 4Z7, Canada

[2] Univ New Brunswick, Fac Forestry & Environm Management, Fredericton, NB E3B 6C2, Canada

[3] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China

[4] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA

来源：

MOLECULAR BREEDING | 2010年 / 25卷 / 01期

关键词：

Potato; High-resolution DNA melting; Haplotype dosage; Autotetraploid genetics; Marker-assisted selection; Variant scanning; MARKER-ASSISTED SELECTION; LATE BLIGHT RESISTANCE; CLOSED-TUBE; PATHOGEN RESISTANCE; GENE; PCR; IDENTIFICATION; POLYMORPHISM; GENOME; ALLELE;

D O I：

10.1007/s11032-009-9309-4

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The ideal marker system for tetraploid potato would be dosage-sensitive and have the ability to distinguish heterozygous genotypes with multiple haplotypes within the genomic region targeted by the marker. The objective of this study was to evaluate the utility of high-resolution DNA melting (HRM) for genotyping and polymorphism detection in diploid and tetraploid potato. Amplicon scanning, unlabelled probe, and short amplicon assays were developed for four candidate genes affecting tuber skin and flesh colour, and starch, and a marker linked to nematode resistance. Genotyping a set of 95 potato clones revealed several examples of clones with three distinct haplotypes. Combined probe and amplicon analysis identified between 29 and 44 unique genotypes for the same assays. Assays developed for four of the five target genes are suitable for marker-assisted selection in potato breeding programs. This study illustrates the use of HRM in potato genetics. Further advances in the technology and associated data analysis should make HRM a useful tool for basic and applied studies of potato.

引用

页码：67 / 90

页数：24

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