Activities of gastric, pancreatic, and intestinal brush-border membrane enzymes during postnatal development of dogs

被引:18
作者
Buddington, RK [1 ]
Elnif, J
Malo, C
Donahoo, JB
机构
[1] Mississippi State Univ, Coll Arts & Sci, Dept Biol Sci, Mississippi State, MS 39762 USA
[2] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA
[3] Royal Vet & Agr Univ, Dept Anim Sci & Anim Hlth, DK-1870 Frederiksberg, Denmark
[4] Univ Montreal, Fac Med, Dept Physiol, Membrane Transport Res Grp, Montreal, PQ H3C 3J7, Canada
关键词
D O I
10.2460/ajvr.2003.64.627
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Objective-To measure activities of digestive enzymes during postnatal development in dogs. Sample Population-Gastrointestinal tract tissues obtained from 110 Beagles ranging from neonatal to adult dogs. Procedure-Pepsin and lipase activities were measured in gastric contents, and amylase, lipase, trypsin, and chymotrypsin activities were measured in small intestinal contents and pancreatic tissue. Activities of lactase, sucrase, 4 peptidases, and enteropeptidase were assayed in samples of mucosa obtained from 3 regions of the small intestine. Results-Gastric pH was low at all ages. Pepsin was not detected until day 21, and activity increased between day 63 and adulthood. Activities of amylase and lipase in contents of the small intestine and pancreatic tissue were lower during suckling than after weaning. Activities of trypsin and chymotrypsin did not vary among ages for luminal contents, whereas activities associated with pancreatic tissue decreased between birth and adulthood for trypsin but increased for chymotrypsin. Lactase and gamma-glutamyltranspeptidase activities were highest at birth, whereas the activities of sucrase and the 4 peptidases increased after birth. Enteropeptidase was detected only in the proximal region of the small intestine at all ages. Conclusions and Clinical Relevance-Secretions in the gastrointestinal tract proximal to the duodenum, enzymes in milk, and other digestive mechanisms compensate for low luminal activities of pancreatic enzymes during the perinatal period. Postnatal changes in digestive secretions influence nutrient availability, concentrations of signaling molecules, and activity of antimicrobial compounds that inhibit pathogens. Matching sources of nutrients to digestive abilities will improve the health of dogs during development.
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页码:627 / 634
页数:8
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