Visualizing the Reaction Coordinate of an O-GlcNAc Hydrolase

被引：71

作者：

He, Yuan ^{[1
]}

Macauley, Matthew S. ^{[2
]}

Stubbs, Keith A. ^{[2
,3
]}

Vocadlo, David J. ^{[2
]}

Davies, Gideon J. ^{[1
]}

机构：

[1] Univ York, Dept Chem, York Struct Biol Lab, York YO10 5YW, N Yorkshire, England

[2] Simon Fraser Univ, Dept Chem, Burnaby, BC V5A 1S6, Canada

[3] Univ Western Australia, Sch Biomed Biomol & Chem Sci, Crawley, WA 6009, Australia

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 06期

基金：

英国生物技术与生命科学研究理事会; 加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

STRUCTURAL INSIGHTS; CATALYTIC MECHANISM; PHOSPHORYLATION; GLCNACYLATION; INHIBITORS; DISEASE; MOTION; TAU;

D O I：

10.1021/ja9086769

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

N-Acetylglucosamine beta-O-linked to serine and threonine residues of nucleocytoplasmic proteins (O-GlcNAc) has been linked to neurodegeneration, cellular stress response. and transcriptional regulation Removal of O-GlcNAc is catalyzed by O-GlcNAcase (OGA) Using, a substrate-assisted catalytic mechanism. Here we define the reaction coordinate Using chemical approaches and directly observe both a Michaelis complex and the oxazoline intermediate.

引用

页码：1807 / +

页数：5

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