Steroid-inducible transcription of the 3β/17β-hydroxysteroid dehydrogenase gene (3β/17β-hsd) in Comamonas testosteroni

被引：30

作者：

Cabrera, JE

Paz, JLP

Genti-Raimondi, S ^{[1
]}

机构：

[1] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Bioquim Clin, RA-5000 Cordoba, Argentina

[2] NCI, Dev Genet Sect, Mol Biol Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2000年 / 73卷 / 3-4期

关键词：

D O I：

10.1016/S0960-0760(00)00066-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of the Comamonas testosteroni gene, encoding 3 beta/17 beta-hydroxysteroid dehydrogenase enzyme (3 beta/17 beta-HSD). was analyzed at the transcriptional level. Northern blot analysis detected a 1 kb transcript in bacterial cells grown in minimum media supplemented with Casamino acids and testosterone. Also this transcript was observed when cells were grown in presence of 1-dehydrotestosterone, androstenedione and 1,4-androstadien-3,17dione, but not in presence of acetate, citrate, cholic acid, cholesterol, and cortisol. In addition, this effect was dependent on the presence of another carbon source in the growth medium used, revealing catabolite repression. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：147 / 152

页数：6

共 19 条

[1] CLONING, SEQUENCING AND EXPRESSION OF PSEUDOMONAS-TESTOSTERONI GENE ENCODING 3-ALPHA-HYDROXYSTEROID DEHYDROGENASE [J].