Molecular cloning and expression of cDNA for human RNase H

被引：56

作者：

Wu, HJ ^{[1
]}

Lima, WF ^{[1
]}

Crooke, ST ^{[1
]}

机构：

[1] ISIS Pharmaceut, Dept Mol Pharmacol, Carlsbad, CA 92008 USA

来源：

ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT | 1998年 / 8卷 / 01期

关键词：

D O I：

10.1089/oli.1.1998.8.53

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned, expressed, and purified to electrophoretic homogeneity a human RNase H. The enzyme has a molecular weight of 32 kDa, is Mg2+ dependent, and is inhibited by Mn2+ and N-ethylmaleimide. Its molecular weight and cleavage characteristics are consistent with type 2 human RNase H. The human RNase H we have cloned is highly homologous to Escherichia coli RNase HI (33.6% amino acid identity) and to other RNase H enzymes homologous to E. coli RNase HI. The enzyme is encoded by a single gene that is at least 10 kb in length and is expressed ubiquitously in human cells and tissues.

引用

页码：53 / 61

页数：9

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