Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis

被引：46

作者：

Kjelgaard-Petersen, Cecilie ^{[1
,3
]}

Siebuhr, Anne Sofie ^{[1
]}

Christiansen, Thorbjorn ^{[2
]}

Ladel, Christoph ^{[1
]}

Karsdal, Morten ^{[1
]}

Bay-Jensen, Anne-Christine ^{[1
]}

机构：

[1] Nord Biosci, Dept Rheumatol Biomarkers & Res, Herlev Hovedgade 207, DK-2730 Herlev, Denmark

[2] Gentofte Univ Hosp, Orthopaed Surg Unit, Gentofte, Denmark

[3] Tech Univ Denmark, Dept Syst Biol, Soltofts Plads Bygning 221, DK-2800 Lyngby, Denmark

来源：

BIOMARKERS | 2015年 / 20卷 / 08期

关键词：

synovial membrane; personalized medicine; Neo-epitope biomarkers; FIBROBLAST-LIKE SYNOVIOCYTES; GROWTH-FACTOR; CARTILAGE; EXPRESSION; MACROPHAGES; CYTOKINE; MARKER; CELLS; MMP-3; RESPONSES;

D O I：

10.3109/1354750X.2015.1105497

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Objective: Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Methods: Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium. Results: TNF alpha significantly increased C1M up to seven-fold (p = 0.0002), C3M up to 24-fold (p = 0.0011), and acMMP3 up to 14-fold (p < 0.0001) in SMEs. IL-1 beta also significantly increased C1M up to five-fold (p = 0.00094), C3M four-fold (p = 0.007), and acMMP3 18-fold (p < 0.0001) in SMEs. Conclusion: The biomarkers C1M, C3M, and acMMP-3 were synovitis biomarkers ex vivo and provide a translational tool together with the SME model.

引用

页码：547 / 556

页数：10

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