Effect of substrate concentration on the enantioselectivity of cyclohexanone monooxygenase from Acinetobacter calcoaceticus and its rationalization

被引：22

作者：

Zambianchi, F

Pasta, P

Ottolina, G

Carrea, G

Colonna, S

Gaggero, N

Ward, JM

机构：

[1] CNR, Ist Biocatalisi & Riconoscimento Mol, I-20131 Milan, Italy

[2] Ist Chim Organ, Fac Farm, I-20133 Milan, Italy

[3] Ctr CNR, I-20133 Milan, Italy

[4] UCL, Dept Biochem & Mol Biol, London WC1E 6BT, England

来源：

TETRAHEDRON-ASYMMETRY | 2000年 / 11卷 / 18期

关键词：

D O I：

10.1016/S0957-4166(00)00354-2

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The ee values of lactone 3, and not of lactone 2, obtained from the enantiodivergent oxidation of racemic bicyclo[3.2.0]hept-2-en-6-one 1, catalyzed by cyclohexanone monooxygenase from Acinetobacter calcoaceticus, were found to be markedly dependent on the degree of conversion and substrate concentration. The results are rationalized on the basis of a model which hypothesizes the binding of a second substrate molecule to an enzyme site distinct from the catalytic site. (C) 2000 Elsevier Science Ltd. All rights reserved.

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页码：3653 / 3657

页数：5

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