RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation

被引：390

作者：

Mazan-Mamczarz, K

Galbán, S

de Silanes, IL

Martindale, JL

Atasoy, U

Keene, JD

Gorospe, M

机构：

[1] NIA, Intramural Res Program, Cellular & Mol Biol Lab, NIH, Baltimore, MD 21224 USA

[2] Duke Univ, Sch Med, Durham, NC 27710 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2003年 / 100卷 / 14期

关键词：

UV light; embryonic lethal abnormal vision;

D O I：

10.1073/pnas.1432104100

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Exposure to short-wavelength UV light (UVC) strongly induces p53 expression. In human RKO colorectal carcinoma cells, this increase was not due to elevated p53 mRNA abundance, cytoplasmic export of p53 mRNA or UVC-triggered stabilization of the p53 protein. Instead, p53 translation was potently enhanced after UVC irradiation. The 3' UTR of p53 was found to be a target of the RNA-binding protein HuR in a UVC-dependent manner in vitro and in vivo. HuR-overexpressing RKO cells displayed elevated p53 levels, whereas cells expressing reduced HuR showed markedly diminished p53 abundance and p53 translation. Our results demonstrate a role for HuR in binding to the p53 mRNA and enhancing its translation.

引用

页码：8354 / 8359

页数：6

共 29 条

[1] ELAV tumor antigen, Hel-N1, increases translation of neurofilament M mRNA and induces formation of neurites in human teratocarcinoma cells [J].