Activity of wild-type and hybrid Bacillus thuringiensis δ-endotoxins against Agrotis ipsilon

被引：24

作者：

de Maagd, RA

Weemen-Hendriks, M

Molthoff, JW

Naimov, S

机构：

[1] Plant Res Int BV, NL-6700 AA Wageningen, Netherlands

[2] Paisij Hilendarski Univ Plovdiv, Dept Mol Biol & Plant Physiol, BG-4000 Plovdiv, Bulgaria

来源：

ARCHIVES OF MICROBIOLOGY | 2003年 / 179卷 / 05期

关键词：

Bacillus thuringiensis; delta-endotoxin; hybrid protein; Agrotis ipsilon;

D O I：

10.1007/s00203-003-0543-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Twelve Cry1 and two Cry9 delta-endotoxins from Bacillus thuringiensis were tested for their activity against black cutworm (Agrotis ipsilon). A. ipsilon was not susceptible to many toxins, but three toxins had significant activity. Cry9Ca was the most toxic, followed by Cry1Aa and Cry1Fb. Hybrids between these three active proteins were made by in vivo recombination and analyzed for activity against A. ipsilon. Analysis of hybrids between Cry1Aa and Cry1Fb indicated that domain 1 of Cry1Aa protein was involved in its higher activity.

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收藏

页码：363 / 367

页数：5

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