Structural study of the exopolysaccharide produced by a clinical isolate of Burkholderia cepacia

被引：57

作者：

Cescutti, P

Bosco, M

Picotti, F

Impallomeni, G

Leitao, JH

Richau, JA

Sá-Correia, I

机构：

[1] Univ Trieste, Dipartimento Biochim Biofis & Chim Macromol, I-34127 Trieste, Italy

[2] POLY Tech Scarl, Area Ric, I-34012 Trieste, Italy

[3] CNR, Ist Chim & Tecnol Mat Polimerici, I-95125 Catania, Italy

[4] Inst Super Tecn, Ctr Engn Biol & Quim, P-1049001 Lisbon, Portugal

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 273卷 / 03期

关键词：

Burkholderia cepacia; cystic fibrosis; exo-polysaccharide; structure; NMR; mass spectrometry;

D O I：

10.1006/bbrc.2000.3059

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The primary structure of the exopolysaccharide produced by a clinical isolate of the bacterium Burkholderia cepacia was studied by means of methylation analysis, selective degradation, NMR spectroscopy, and electrospray mass spectrometry, The resulting data showed that the parent repeating unit of the exopolysaccharide is a highly branched heptasaccharide with the following structure: [GRAPHICS] Two acetyl groups are present per repeating unit, as noncarbohydrate substituents, (C) 2000 Academic Press.

引用

页码：1088 / 1094

页数：7

共 24 条

[11] Cerantola S, 1996, CARBOHYD RES, V285, P59, DOI 10.1016/S0008-6215(96)90170-6
[12] Exopolysaccharide production by mucoid and non-mucoid strains of Burkholderia cepacia
Cérantola, S
Bounéry, JD
Segonds, C
Marty, N
Montrozier, H
[J]. FEMS MICROBIOLOGY LETTERS, 2000, 185 (02) : 243 - 246
[13] Structural investigation of the exopolysaccharide produced by Pseudomonas flavescens strain B62 - Degradation by a fungal cellulase and isolation of the oligosaccharide repeating unit
Cescutti, P
Toffanin, R
Fett, WF
Osman, SF
Pollesello, P
Paoletti, S
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 251 (03): : 971 - 979
[14] CESCUTTI P, 1992, EUR J BIOCHEM, V213, P445
[15] THE APPLICATION OF MAXIMUM-ENTROPY PROCESSING TO THE DECONVOLUTION OF COUPLING PATTERNS IN NMR
DELSUC, MA
LEVY, GC
[J]. JOURNAL OF MAGNETIC RESONANCE, 1988, 76 (02): : 306 - 315
[16] DELSUC MA, 1989, MAXIMUM ENTROPY BAYE
[17] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES
DUBOIS, M
GILLES, KA
HAMILTON, JK
REBERS, PA
SMITH, F
[J]. ANALYTICAL CHEMISTRY, 1956, 28 (03) : 350 - 356
[18] DUDMAN WF, 1983, CARBOHYD RES, V117, P141, DOI 10.1016/0008-6215(83)88083-5
[19] LINBERG B, 1973, CARBOHYD RES, V28, P351
[20] Molecular typing and exopolysaccharide biosynthesis of Burkholderia cepacia isolates from a Portuguese Cystic Fibrosis Center
Richau, JA
Leitao, JH
Correia, M
Lito, L
Salgado, MJ
Barreto, C
Cescutti, P
Sá-Correia, I
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (04) : 1651 - 1655

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